Helicase SRCAP (Snf2-related CBP activator, domino homolog 2, SRCAP, KIAA0309)

Function: - catalytic component of the SRCAP complex - acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, & Notch-mediated transcription - interacts with CREBBP & EP300 - may be part of a complexcontaining SRCAP, CREBBP, CARM1 & GRIP1 - interacts with hepatitis C virus (HCV) NS5A & human adenovirus 2 DBP - phosphorylated upon DNA damage, probably by ATM or ATR Kinetic parameters: - KM=66 uM for ATP Structure: - belongs to the SNF2/RAD54 helicase family, SWR1 subfamily - contains 3 A.T hook DNA-binding domain - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain - contains 1 HSA domain Compartment: nucleus Alternative splicing: named isoforms=3

General

helicase (DNA unwinding protein, DNA untwisting protein) phosphoprotein

Properties

SIZE: entity length = 3230 aa MW = 344 kD COMPARTMENT: cell nucleus MOTIF: HSA {124-196} Ser phosphorylation site {S248} proline-rich region SITE: 275-278 MOTIF: proline residue (SEVERAL) glutamate-rich region {293-563} MOTIF: glutamate residue (SEVERAL) helicase NAME: helicase SITE: 630-795 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 643-650 proline-rich region SITE: 981-1883 MOTIF: proline residue (SEVERAL) active site MOTIF: P-loop FOR-BINDING-OF: adenosine triphosphate Helicase C-terminal {2044-2197} glutamate-rich region {2227-2362} MOTIF: glutamate residue (SEVERAL) Thr phosphorylation site {T2258} proline-rich region SITE: 2426-3023 MOTIF: proline residue (SEVERAL) A+T hook SITE: 2857-2869 Ser phosphorylation site {S2869} A+T hook SITE: 2936-2948 A+T hook SITE: 3004-3016 Ser phosphorylation site {S3148} glutamate-rich region {3179-3182} MOTIF: glutamate residue (SEVERAL)

Database Correlations

UniProt Q6ZRS2 PFAM correlations

References

UniProt :accession Q6ZRS2