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sputum gram stain
Indications:
- lower respiratory tract infection
Specimen:
1) sputum (not saliva)
2) minimum volume: 2 mL
3) container:
-> commercially available sputum collection system or similar sterile container with a screw cap
Notes:
1) > 10 epithelial cells/low power field suggests oropharyngeal specimen
2) < 10 epithelial cells per low power field (LPF) & leukocytes > 25 per LPF plus the findings of bacteria on gram stain support a diagnosis of bacterial pneumonia [2] & the need for sputum culture
Related
culture of sputum
sputum (phlegm)
General
Gram stain
References
- Harrison's Principles of Internal Medicine, 13th ed.
Isselbacher et al (eds), McGraw-Hill Inc. NY, 1994, pg 491
- Medical Knowledge Self Assessment Program (MKSAP) 15,
American College of Physicians, Philadelphia 2009