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C-Jun-amino-terminal kinase-interacting protein 4; JIP-4; JNK-interacting protein 4; cancer/testis antigen 89; CT89; human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; mitogen-activated protein kinase 8-interacting protein 4; proliferation-inducing protein 6; protein highly expressed in testis; PHET; sperm surface protein; sperm-associated antigen 9; sperm-specific protein; Sunday driver 1 (SPAG9, HSS, KIAA0516, MAPK8IP4, SYD1, HLC6)
Function:
- the JNK-interacting protein (JIP) group of scaffold proteins selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module
- interacts with MAX, MAPK8, MAPK9, MAPK10,MAPK14, MAP3K3, MYC, KNS2, MAP2K4
- interaction with KNS2 is important in the formation of ternary complex with MAPK8
- interacts with NFKB1
- phosphorylated by MAPK8 & MAPK14
- phosphorylated upon DNA damage, probably by ATM or ATR
Structure:
- homo-oligomer
- belongs to the JIP scaffold family
Compartment:
- cytoplasm
- perinuclear distribution in response to stress signals such as UV radiation
- isoform 5:
- cytoplasmic vesicle, secretory vesicle, acrosome
- associated with the plasma membrane of the acrosome
- also localizes in the acrosome matrix
Alternative splicing: named isoforms=8
Expression:
- isoform 5 is expressed only in testis
- expressed in round spermatids of stages 1-3
- absent in spermatogonia & spermatocytes
- isoform 3
- expressed only in testis
- expression is increased in systemic sclerosis fibroblasts
- isoform 4 is expressed in testis & in acute myeloid leukemia
General
c-jun-amino-terminal kinase interacting protein (JNK-interacting protein, JIP, JNK MAP kinase scaffold protein)
phosphoprotein
Properties
SIZE: entity length = 1321 aa
MW = 146 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
coiled coil {66-166}
MOTIF: Ser phosphorylation site {S109}
Ser phosphorylation site {S183}
Ser phosphorylation site {S185}
Ser phosphorylation site {S203}
Thr phosphorylation site {T217}
Thr phosphorylation site {T226}
Ser phosphorylation site {S238}
Thr phosphorylation site {T244}
Ser phosphorylation site {S265}
Ser phosphorylation site {S268}
Thr phosphorylation site {T276}
Thr phosphorylation site {T292}
Ser phosphorylation site {S311}
Ser phosphorylation site {S329}
Ser phosphorylation site {S332}
Thr phosphorylation site {T365}
Ser phosphorylation site {S387}
Ser phosphorylation site {S388}
Ser phosphorylation site {S391}
coiled coil {408-534}
MOTIF: Thr phosphorylation site {T418}
Thr phosphorylation site {T586}
Ser phosphorylation site {S594}
Thr phosphorylation site {T595}
coiled coil {724-758}
MOTIF: Ser phosphorylation site {S728}
Ser phosphorylation site {S730}
Ser phosphorylation site {S732}
Ser phosphorylation site {S733}
Ser phosphorylation site {S1144}
Thr phosphorylation site {T1264}
Database Correlations
OMIM 605430
UniProt O60271
Pfam PF09744
Entrez Gene 9043
Kegg hsa:9043
References
UniProt :accession O60271