SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; sucrose nonfermenting protein 2 homolog; hSNF2H (SMARCA5, SNF2H, WCRF135)

Function: - helicase with intrinsic ATP-dependent nucleosome-remodeling activity - complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails - also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A - probably plays a role in repression of RNA polymerase 1 dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter - component of the WICH complex - component of the nucleolar remodeling complex (NoRC) - catalytic subunit of chromatin-remodeling complexes: - CHRAC complex, RSF complex, ACF/WCRF complex, & WICH complex - each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5 - ACF/WCRF complex contains BAZ1A - CHRAC complex contains BAZ1A, CHRAC1, & POLE3 - RSF complex contains RSF1 - WICH complex contains BAZ1B/WSTF - SMARCA5 is the catalytic subunit of the NoRC complex, which also contains BAZ2A/TIP5 - catalytic subunit of SMARCA5/cohesin/NuRD complexes - interacts with MYO1C (putative) Structure: - belongs to the SNF2/RAD54 helicase family, ISWI subfamily - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain - contains 2 SANT domains Compartment: nucleus Expression: ubiquitously expressed Pathology: - overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia - down-regulation correlates with hematologic remission following chemotherapy

General

DEAD box ATP-dependent helicase phosphoprotein SWI/SNF-related matrix-associated actin dependent regulator of chromatin subfamily A

Properties

SIZE: entity length = 1052 aa MW = 122 kD COMPARTMENT: cell nucleus MOTIF: proline-rich region SITE: 7-13 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S66} Thr phosphorylation site {T113} Thr phosphorylation site {T115} Ser phosphorylation site {S116} Ser phosphorylation site {S137} helicase NAME: helicase SITE: 192-357 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 205-212 DEAD/H box NAME: DEAD/H box SITE: 308-311 Helicase C-terminal {487-638} Ser phosphorylation site {S699} Ser phosphorylation site {S755} SANT 1 {840-892} SANT 2 {943-1007}

Database Correlations

OMIM 603375 UniProt O60264 PFAM correlations Entrez Gene 8467 Kegg hsa:8467

References

Uniprot :accession O60264