Slit homolog 2 protein (SLIT2, SLIL3)

Function: 1) molecular guidance cue in cellular migration 2) interaction with roundabout homolog receptors 3) axonal navigation at ventral midline of neural tube 4) projection of axons to different regions 5) midline guidance in the forebrain - repulsive signal preventing inappropriate midline crossing by axons projecting from olfactory bulb 6) in spinal cord, guides commissural axons once they reached the floor plate via response to netrin 7) silences the attractive effect of NTN1 but not its growth-stimulatory effect (in vitro) 8) spinal cord midline post-crossing axon repulsion (putative) 9) repellent for retinal ganglion axons in developing visual system - collapses & repels retinal ganglion cell growth cones 10) branching & arborization of CNS sensory axons 11) neuronal cell migration 12) leukocyte migration 13) interacts with GREM1, ROBO1, ROBO₂ Structure: - the leucine-rich repeat domain is sufficient for guiding both axon projection & neuronal migration, in vitro -contains 1 CTCK (C-terminal cystine knot-like) domain - contains 7 EGF-like domains - contains 1 laminin G-like domain - contains 20 LRR repeats (leucine-rich repeats) - contains 4 LRRCT domains - contains 4 LRRNT domains Compartment: - secreted - the C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated Alternative splicing: named isoforms=3 Expression: - expressed in fetal lung, kidney, adult spinal cord > adult adrenal gland, thyroid, trachea

General

leucine-rich repeat-containing protein (LRRC) multiple epidermal growth factor like domains (protein) slit homolog protein

Properties

SIZE: entity length = 1529 aa MW = 170 kD COMPARTMENT: extracellular compartment MOTIF: signal sequence {1-30} LRRNT {31-55} leucine-rich repeat SITE: 56-77 MOTIF: leucine residue (SEVERAL) N-glycosylation site {N66} leucine-rich repeat SITE: 80-101 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 104-125 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 128-149 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 152-173 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 176-197 MOTIF: leucine residue (SEVERAL) N-glycosylation site {N186} LRRCT 1 {209-259} LRRNT 2 {264-300} MOTIF: cysteine residue {C277} MODIFICATION: cysteine residue {C286} cysteine residue {C286} MODIFICATION: cysteine residue {C277} leucine-rich repeat SITE: 301-322 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 325-346 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 349-370 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 373-394 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 397-418 MOTIF: leucine residue (SEVERAL) LRRCT 2 {430-480} MOTIF: cysteine residue {C434} MODIFICATION: cysteine residue {C457} cysteine residue {C436} MODIFICATION: cysteine residue {C478} cysteine residue {C457} MODIFICATION: cysteine residue {C434} cysteine residue {C478} MODIFICATION: cysteine residue {C436} LRRNT 3 {497-533} MOTIF: cysteine residue {C506} MODIFICATION: cysteine residue {C512} cysteine residue {C510} MODIFICATION: cysteine residue {C519} cysteine residue {C512} MODIFICATION: cysteine residue {C506} cysteine residue {C519} MODIFICATION: cysteine residue {C510} leucine-rich repeat SITE: 534-555 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 559-580 MOTIF: leucine residue (SEVERAL) N-glycosylation site {N564} leucine-rich repeat SITE: 583-604 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 607-628 MOTIF: leucine residue (SEVERAL) N-glycosylation site {N623} leucine-rich repeat SITE: 631-652 MOTIF: leucine residue (SEVERAL) LRRCT 3 {664-714} MOTIF: cysteine residue {C668} MODIFICATION: cysteine residue {C691} cysteine residue {C670} MODIFICATION: cysteine residue {C712} cysteine residue {C691} MODIFICATION: cysteine residue {C668} cysteine residue {C712} MODIFICATION: cysteine residue {C670} LRRNT 4 {718-754} MOTIF: cysteine residue {C727} MODIFICATION: cysteine residue {C733} cysteine residue {C731} MODIFICATION: cysteine residue {C740} cysteine residue {C733} MODIFICATION: cysteine residue {C727} cysteine residue {C740} MODIFICATION: cysteine residue {C731} leucine-rich repeat SITE: 755-777 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 778-799 MOTIF: leucine residue (SEVERAL) N-glycosylation site {N794} N-glycosylation site {N799} leucine-rich repeat SITE: 802-823 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 826-847 MOTIF: leucine residue (SEVERAL) LRRCT 4 {859-909} MOTIF: cysteine residue {C863} MODIFICATION: cysteine residue {C886} cysteine residue {C865} MODIFICATION: cysteine residue {C907} cysteine residue {C886} MODIFICATION: cysteine residue {C863} cysteine residue {C907} MODIFICATION: cysteine residue {C865} EGF domain {918-955} MOTIF: cysteine residue {C922} MODIFICATION: cysteine residue {C933} cysteine residue {C927} MODIFICATION: cysteine residue {C943} cysteine residue {C933} MODIFICATION: cysteine residue {C922} cysteine residue {C943} MODIFICATION: cysteine residue {C927} cysteine residue {C945} MODIFICATION: cysteine residue {C954} cysteine residue {C954} MODIFICATION: cysteine residue {C945} EGF domain {957-996} MOTIF: cysteine residue {C961} MODIFICATION: cysteine residue {C972} cysteine residue {C966} MODIFICATION: cysteine residue {C984} cysteine residue {C972} MODIFICATION: cysteine residue {C961} cysteine residue {C984} MODIFICATION: cysteine residue {C966} cysteine residue {C986} MODIFICATION: cysteine residue {C995} cysteine residue {C995} MODIFICATION: cysteine residue {C986} EGF domain {998-1034} MOTIF: cysteine residue {C1002} MODIFICATION: cysteine residue {C1013} cysteine residue {C1007} MODIFICATION: cysteine residue {C1022} N-glycosylation site {N1009} N-glycosylation site {N1010} cysteine residue {C1013} MODIFICATION: cysteine residue {C1002} N-glycosylation site {N1019} cysteine residue {C1022} MODIFICATION: cysteine residue {C1007} cysteine residue {C1024} MODIFICATION: cysteine residue {C1033} cysteine residue {C1033} MODIFICATION: cysteine residue {C1024} EGF domain {1036-1074} MOTIF: cysteine residue {C1040} MODIFICATION: cysteine residue {C1053} cysteine residue {C1047} MODIFICATION: cysteine residue {C1062} cysteine residue {C1053} MODIFICATION: cysteine residue {C1040} cysteine residue {C1062} MODIFICATION: cysteine residue {C1047} cysteine residue {C1064} MODIFICATION: cysteine residue {C1073} cysteine residue {C1073} MODIFICATION: cysteine residue {C1064} EGF domain {1076-1112} MOTIF: cysteine residue {C1080} MODIFICATION: cysteine residue {C1091} cysteine residue {C1085} MODIFICATION: cysteine residue {C1100} cysteine residue {C1091} MODIFICATION: cysteine residue {C1080} cysteine residue {C1100} MODIFICATION: cysteine residue {C1085} cysteine residue {C1102} MODIFICATION: cysteine residue {C1111} cysteine residue {C1111} MODIFICATION: cysteine residue {C1102} EGF domain {1121-1157} MOTIF: proteolytic site {1121-1122} cysteine residue {C1125} MODIFICATION: cysteine residue {C1136} cysteine residue {C1130} MODIFICATION: cysteine residue {C1145} cysteine residue {C1136} MODIFICATION: cysteine residue {C1125} cysteine residue {C1145} MODIFICATION: cysteine residue {C1130} cysteine residue {C1147} MODIFICATION: cysteine residue {C1156} cysteine residue {C1156} MODIFICATION: cysteine residue {C1147} Laminin G-like {1160-1333} MOTIF: N-glycosylation site {N1183} N-glycosylation site {N1266} N-glycosylation site {N1300} cysteine residue {C1307} MODIFICATION: cysteine residue {C1333} EGF domain {1332-1368} MOTIF: cysteine residue {C1333} MODIFICATION: cysteine residue {C1307} cysteine residue {C1336} MODIFICATION: cysteine residue {C1346} cysteine residue {C1341} MODIFICATION: cysteine residue {C1356} cysteine residue {C1346} MODIFICATION: cysteine residue {C1336} cysteine residue {C1356} MODIFICATION: cysteine residue {C1341} cysteine residue {C1358} MODIFICATION: cysteine residue {C1367} cysteine residue {C1367} MODIFICATION: cysteine residue {C1358} cysteine residue {C1375} MODIFICATION: cysteine residue {C1385} cysteine residue {C1380} MODIFICATION: cysteine residue {C1395} cysteine residue {C1385} MODIFICATION: cysteine residue {C1375} cysteine residue {C1395} MODIFICATION: cysteine residue {C1380} cysteine residue {C1397} MODIFICATION: cysteine residue {C1406} cysteine residue {C1406} MODIFICATION: cysteine residue {C1397} cysteine residue {C1416} MODIFICATION: cysteine residue {C1426} cysteine residue {C1421} MODIFICATION: cysteine residue {C1436} cysteine residue {C1426} MODIFICATION: cysteine residue {C1416} cysteine residue {C1436} MODIFICATION: cysteine residue {C1421} cysteine residue {C1438} MODIFICATION: cysteine residue {C1447} cysteine residue {C1447} MODIFICATION: cysteine residue {C1438} C-terminal cystine knot {1453-1528} MOTIF: cysteine residue {C1453} MODIFICATION: cysteine residue {C1492} cysteine residue {C1471} MODIFICATION: cysteine residue {C1506} cysteine residue {C1482} MODIFICATION: cysteine residue {C1522} cysteine residue {C1486} MODIFICATION: cysteine residue {C1524} cysteine residue {C1492} MODIFICATION: cysteine residue {C1453} cysteine residue {C1506} MODIFICATION: cysteine residue {C1471} cysteine residue {C1522} MODIFICATION: cysteine residue {C1482} cysteine residue {C1524} MODIFICATION: cysteine residue {C1486}

Database Correlations

OMIM 603746 UniProt O94813 PFAM correlations Entrez Gene 9353 Kegg hsa:9353

References

UniProt :accession O94813