testis anion transporter 1; anion exchange transporter; solute carrier family 26 member 8 (SLC26A8, TAT1)

Function: - acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate & oxalate transport. - may fulfill critical anion exchange functions in male germ line during meiosis & hence may play a role in spermatogenesis - may be involved in a new regulatory pathway linking sulfate transport to RhoGTPase signaling in male germ cells - critical component of the sperm annulus that is essential for correct sperm tail differentiation & motility & hence male fertility - interacts with RACGAP1 Inhibition: - repressed by tunicamycin, an inhibitor of N-glycosylation - activity is inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS) - an inhibitor of several anionic channels & transporters, gluconate, & by thiosulfate Structure: - N-glycosylated - belongs to the SLC26A/sulP transporter (TC 2.A.53) family - contains 1 STAS domain Compartment: - membrane - located at the annulus ring structure within the sperm cell Alternative splicing: named isoforms=4 Expression: - expression observed exclusively in testis - restricted to the meiotic phase of the germ cell - abundant expression located in the seminiferous tubules, concentrated on the luminal side of the tubuli harboring the spermatocytes & spermatids - expressed in spermatozoa

General

glycoprotein solute carrier family 26 (SLC26, SLC26A) testis-specific protein transmembrane 12 protein

Properties

SIZE: entity length = 970 aa MW = 109 kD COMPARTMENT: cellular membrane MOTIF: cytoplasmic domain {1-95} transmembrane domain {96-116} exoplasmic loop {117-119} transmembrane domain {120-140} cytoplasmic loop {141-146} transmembrane domain {147-167} exoplasmic loop {168-202} MOTIF: N-glycosylation site {N192} transmembrane domain {203-223} cytoplasmic loop {224-232} transmembrane domain {233-253} exoplasmic loop {254-270} transmembrane domain {271-291} cytoplasmic loop {292-307} transmembrane domain {308-328} exoplasmic loop {329-355} transmembrane domain {356-376} cytoplasmic loop {377-392} transmembrane domain {393-413} exoplasmic loop {414-429} transmembrane domain {430-450} cytoplasmic loop {451-452} transmembrane domain {453-473} exoplasmic loop {474-497} transmembrane domain {498-518} cytoplasmic domain {519-970} MOTIF: STAS {543-795} RACGAP1 interaction {664-970}

Database Correlations

OMIM 608480 UniProt Q96RN1 PFAM correlations Kegg hsa:1163

References

UniProt :accession Q96RN1