S-phase kinase-associated protein 2 selections

S-phase kinase-associated protein 2; F-box protein Skp2; cyclin-A/CDK2-associated protein p45; p45skp2; F-box/LRR-repeat protein 1 (SKP2 FBXL1)

Function: - substrate recognition component of a SCF complex which mediates ubiquitination & subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction & transcription - specifically recognizes phosphorylated CDKN1B/p27kip - role in regulation of G1/S transition - degradation of CDKN1B/p27kip also requires CKS1 recognizes target proteins ORC1L, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, & probably MYC, TOB1 & TAL1 - degradation of TAL1 also requires STUB1 - recognizes CDKN1A in association with CCNE1 or CCNE2 & CDK2 - interacts directly with CUL1 & SKP1 - interacts with CKS1 - interacts with the cyclin-A-CDK2 complex - interacts with ORC1L, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 & MLL Structure: - contains 1 F-box domain - contains 8 LRR repeats (leucine-rich repeats) Alternative splicing: named isoforms=2

General

leucine-rich repeat-containing protein (LRRC) phosphoprotein Skp (S phase kinase associated protein) family protein

Properties

SIZE: entity length = 424 aa MW = 48 kD MOTIF: Ser phosphorylation site {S64} F-box domain {94-140} Ser phosphorylation site {S179} leucine-rich repeat SITE: 180-204 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 210-234 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 258-284 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 286-308 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 309-330 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 334-356 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 359-378 MOTIF: leucine residue (SEVERAL) leucine-rich repeat SITE: 380-403 MOTIF: leucine residue (SEVERAL)

Database Correlations

OMIM 601436 UniProt Q13309 Pfam PF00646 Entrez Gene 6502 Kegg hsa:6502

References

UniProt :accession Q13309
Zhang et al. p19Skp1 and p45Skp2 are essential elements of the cyclin A- CDK2 S phase kinase. Cell 82:915-25 1995 PMID: 7553852

Component-of

SKP1-CUL1-F-box protein (SCF) complex; SCF(FBXW11) E3 ligase complex; CF(SKP2) E3 ligase complex