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SKAP complex; astrin-kinastrin complex; SPAG5-kinastrin complex

Function: - promotes stable microtubule-kinetochore attachments Structure: - subunits include: KNSTRN, SPAG5, PLK1, DYNLL1 & SGOL2 Compartment: - located at the kinetochore-microtubule interface - localizes to the microtubule plus ends

General

molecular complex

Properties

SUBUNITS: small kinetochore-associated protein COMPARTMENT: cytoplasm cell nucleus MOTIF: Ser phosphorylation site {S128} SPAG5 interaction {159-316} MOTIF: coiled coil {166-216} coiled coil {248-316} sperm-associated antigen 5 COMPARTMENT: cytoplasm MOTIF: Ser phosphorylation site {S12} Ser phosphorylation site {S14} Ser phosphorylation site {S62} Ser phosphorylation site {S66} Thr phosphorylation site {T111} Ser phosphorylation site {S135} Ser phosphorylation site {S249} Ser phosphorylation site {S334} Ser phosphorylation site {S341} Ser phosphorylation site {S353} Ser phosphorylation site {S362} Ser phosphorylation site {S364} Thr phosphorylation site {T377} Thr phosphorylation site {T389} Ser phosphorylation site {S401} coiled coil {545-608} glutamine-rich region {617-789} MOTIF: glutamine residue (SEVERAL) coiled coil {759-868} Thr phosphorylation site {T937} coiled coil {979-1174} serine/threonine protein kinase PLK1 COMPARTMENT: cytoplasm cell nucleus MOTIF: Thr phosphorylation site {T6} kinase domain SITE: 53-305 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 59-67 ATP-binding site NAME: ATP-binding site SITE: 82-82 Ser phosphorylation site {S103} ATP-binding site NAME: ATP-binding site SITE: 131-131 Ser phosphorylation site {S137} aspartate residue {D176} ATP-binding site NAME: ATP-binding site SITE: 178-181 Activation loop {194-221} ATP-binding site NAME: ATP-binding site SITE: 194-194 Thr phosphorylation site {T210} Thr phosphorylation site {T214} Ser phosphorylation site {S269} Ser phosphorylation site {S335} ubiquitination site (destruction box, D-box) SITE: 337-340 FOR-BINDING-OF: ubiquitin Ser phosphorylation site {S375} POLO box domain {417-480} MOTIF: Ser phosphorylation site {S450} Ser phosphorylation site {S461} Thr phosphorylation site {T464} Linker {493-507} MOTIF: Thr phosphorylation site {T498} POLO box domain {515-584} MOTIF: domain {538-540} STATE: active state dynein light chain 1 COMPARTMENT: cytoplasm INHIBITS: NO synthase-1 Shugoshin-like 2 COMPARTMENT: cell nucleus MOTIF: coiled coil {69-116} Ser phosphorylation site {S266} coiled coil {452-476} Ser phosphorylation site {S507} coiled coil {603-626} Thr phosphorylation site {T845}

References

UniProt :accession Q9Y448

Components

dynein light chain 1, cytoplasmic (protein inhibitor of neuronal nitric oxide synthase, PIN, DLC1, DYNLL1, DNCL1, DNCLC1, HDLC1) serine/threonine protein kinase PLK1; polo-like kinase 1; PLK-1; serine/threonine protein kinase 13; STPK13 (PLK1 PLK) Shugoshin-like 2 (Tripin) (SGOL2) small kinetochore-associated protein; SKAP; kinetochore-localized astrin-binding protein; kinastrin; kinetochore-localized astrin/SPAG5-binding protein; TRAF4-associated factor 1 (KNSTRN, C15orf23, SKAP, TRAF4AF1, HSD11) sperm-associated antigen 5 (astrin, mitotic spindle-associated protein p126, MAP126, Deepest, SPAG5)