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luteinizing hormone (LH) in serum

Reference values: 1) MALE: 1.4 to 11.1 mIU/mL 2) FEMALE: a) Follicular phase: 1.9 to 11.9 mIU/mL b) Midcycle peak: 16.6 to 81.1 mIU/mL c) Luteal phase: 3.2 to 33.9 mIU/mL 3) CHILDREN (Pre-pubertal): 0.0 to 2.7 mIU/mL Clinical significance: A wide array of medications affect FSH & LH levels. For instance, drugs containing estrogen or progesterone (e.g. oral contraceptives or estrogen replacement drugs) have been reported to decrease levels of FSH & LH. Similarly, central nervous system (CNS) acting psychoactive drugs which affect the hypothalamus also cause decreased FSH & LH levels. Like any analyte used as a diagnostic adjunct. LH results must be interpreted carefully with the overall clinical presentations & other supportive diagnostic tests. Increases: - primary hypogonadism - polycystic ovary syndrome - menopause - pituitary adenoma (rare) Decreases: - pituitary insifficiency - Kallman's syndrome - anorexia nervosa - fertile enuch (isolated LH deficiency) - severe stress - malnutrition - pharmaceutical agents - estrogens - psychoactive medications Principle: The Allegro LH Immunoassay incorporates two monoclonal antibodies with high affinity for LH. The two antibodies, Ab(1) & Ab(2) are selected & prepared to be specific for different & distintic epitopes (sites) on the beta-subunit of the LH molecule. Both antibodies bind without competition or stearic interference from each other, & form a soluble sandwich complex: Ab(1)__LH__Ab(2) One monoclonal antibody is radiolabeled for detection, [Ab(2)*], while the other monoclonal antibody is coupled to biotin [Biotin- Ab(1)]. The addition to the reaction mixture of an avidin coated plastic bead allows for a specific & efficient means of binding the sandwich complex to a solid phase via the high affinity interaction between biotin & avidin. Specimen: 1) Determination of LH should be performed on serum. Collect blood sample in a red-top venipuncture tube & allow blood to clot. Centrifuge the sample & separate from cells. 2) If assay is performed within 24 hours after collection, the specimen should be stored in the refrigerator at 2-8o C. If the testing will be delayed more than 24 hours, the specimen should be frozen. Mix thoroughly after thawing to ensure consistency in the results. Avoid repeated freezing & thawing. 3) Specimens showing particulate matter, erythrocytes, or turbidity should be centrifuged before testing.

Related

luteinizing hormone; lutropin (LH)

Specific

luteinizing hormone (LH) in serum baseline

General

luteinizing hormone (LH) in body fluid

References

  1. Nichols Institute, Immunoassay for the Quantitative Determination of Luteinizing Hormone in Human Serum. Nichols Institute Diagnostics, 1988.
  2. Luteinizing Hormone Laboratory Test Directory ARUP: 70093
  3. Mini Panel of 2 tests: Follicle Stimulating Hormone . Luteinizing Hormone Laboratory Test Directory ARUP: 70193

Component-of

anterior pituitary evaluation panel FSH/LH in serum/plasma gonadotropin-releasing hormone stimulation panel luteinizing hormone/follicle-stimulating hormone in serum/plasma; lutropin/follitropin in serum/plasma; LH/FSH in serum/plasma