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amylase in serum/plasma

Indications: - evaluation of acute pancreatitis Reference values: - Male & Female: Serum: 30- 110 U/L Increases: 1) acute pancreatitis a) serum levels rise within 6-48 hours after onset of acute pancreatitis in about 80% of patients b) values in excess of 4 times the upper limit of normal are highly suggestive of pancreatitis c) activity of amylase in serum generally returns to normal within 3-5 days in patients with milder forms of acute pancreatitis d) elevated serum amylase persisting more than 3-5 days suggests pancreatic pseudocyst or pancreatic necrosis [4] e) use in conjunction with serum lipase improves specificity 2) mumps, parotitis 3) intestinal obstruction 4) intestinal ischemia, intestinal infarction, appendicitis 5) renal failure 6) also see hyperamylasemia 7) pharmaceutical agents: - in vivo effects - cholinergic agents, ethanol, narcotics Decreases: - pharmaceutical agents: - chemical interferences - citrate, oxalate, fluorides Principle: - see amylase in serum & urine Specimen: No special patient preparation is necessary. Sample preparation: Collect the specimen by standard venipuncture technique. Use Lithium or sodium heparin as an anticoagulant for plasma specimens. Plasma values are approximately 20 uL higher than serum values. Urine: Collect timed or random specimens by standard laboratory procedures. Timed specimens are preferred: DO NOT USE URINE CONTAINING CHELATING AGENTS. (e.g. citrates, oxalates, or EDTA) Special Precautions: Serum or Plasma: See Sample Dilution for grossly lipemic specimens. Refrigerate or freeze samples that cannot be analyzed immediately. Do not use calcium-chelating anticoagulants such as oxalates, citrated, & EDTA. Minimum sample size 0.5 milliliters with an optimum size of 1.0 milliliter or larger.

Related

amylase hyperamylasemia lipase in serum

Specific

amylase pancreatic in serum amylase salivary in serum

General

amylase in serum/plasma/blood

References

  1. Kodak Ektachem 700 Analyzer Operator's Manual, Kodak Clinical Products, Rochester, New York.
  2. Kodak Ektachem Slide Package Inserts, Kodak Clinical Products Rochester, New York.
  3. Kodak Ektachem Training Manual, Kodak Clinical Products, Rochester, New York.
  4. Henry's Clinical Diagnosis & Management by Laboratory Methods, 21st edition, McPherson RA & Pincus MR (es), W.B. Saunders Co., Philadelphia, PA. 2007, pg 287
  5. Amylase, Plasma or Serum Laboratory Test Directory ARUP: 20013
  6. Panel of 3 tests Laboratory Test Directory ARUP: 20804
  7. Panel of 3 tests Laboratory Test Directory ARUP: 2004464

Component-of

amylase + triacylglycerol lipase in serum/plasma macroamylase/amylase in serum/plasma pancreatic panel