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fructose-1,6-diphosphate aldolase in serum
Indications:
- none really
- AST, LDH & CK have replaced aldolase
Clinical significance:
- serum creatine kinase generally sufficient
- serum aldolase monitoring not routinely recommended
- increased serum aldolase in the absence of increased serum creatine kinase occurs in eosinophilic fasciitis
Increases:
- Duchenne muscular dystrophy (highest levels)
- dermatomyositis, polymositis
- limb-girdle dystrophy
- myocardial infarction
- viral hepatitis
- cirrhosis
- obstructive jaundice
- prostate cancer
- chronic leukemia
Specimen:
1) serum, avoid hemolysis
2) separate from cells immediately
3) stable at room temperature for 8 hours
4) stable at 4 degrees C for 5 days
Reference interval: 1.0-7.5 U/L (adult)
Principle:
aldolase
fructose-1,6-diphosphate -------> glyceraldehyde-3-phosphate
+ dihydroxyacetone phosphate
triose phosphate
isomerase
dihydroxyacetone phosphate -------> glyceraldehyde-3-phosphate
glyceraldehyde-
3-phosphate
dehydrogenase
glyceraldehyde-3-phosphate -----------> 1,3-diphosphoglycerate
+ NAD+ + H2PO4- + NADH
production of NADH is monitored at 340 nM.
Related
fructose-1,6 diphosphate aldolase or fructose diphosphate aldolase
General
aldolase measurement
References
- Clinical Guide to Laboratory Tests, 3rd edition, NW Tietz
ed, WB Saunders, Philadelphia, 1995
- Clinical Guide to Laboratory Tests, 4th edition, HB Wu ed,
WB Saunders, Philadelphia, 2006
- Aldolase
Laboratory Test Directory ARUP: 20012