amylase-1 (salivary amylase, 1,4-alpha-D-glucan glucanohydrolase 1,salivary alpha-amylase, AMY1)

Function: - endohydrolysis of 1,4-alpha-D-glucosidic linkages in oligosaccharides & polysaccharides Cofactor: - binds 1 Ca⁺² per subunit - binds 1 Cl^- per subunit Structure: - monomer - belongs to the glycosyl hydrolase 13 family Compartment: secreted Laboratory: - amylase salivary in serum Notes: - recently duplicated genes AMY1A, AMY1B, & AMY1C show no differences over a region of 950 base pairs sequenced; thus sequences of the amylase 1A, amylase 1B & amylase 1C proteins may be identical

Specific

amylase-1A; 1,4-alpha-D-glucan glucanohydrolase 1; salivary alpha-amylase (AMY1A, AMY1) amylase-1B (salivary) amylase-1C (salivary)

Properties

SIZE: entity length = 511 aa MW = 58 kD COMPARTMENT: extracellular compartment MOTIF: signal sequence {1-15} cysteine residue {C43} MODIFICATION: cysteine residue {C101} cysteine residue {C85} MODIFICATION: cysteine residue {C130} cysteine residue {C101} MODIFICATION: cysteine residue {C43} Ca+2-binding site SITE: 115-115 cysteine residue {C130} MODIFICATION: cysteine residue {C85} cysteine residue {C156} MODIFICATION: cysteine residue {C175} Ca+2-binding site SITE: 173-173 cysteine residue {C175} MODIFICATION: cysteine residue {C156} Ca+2-binding site SITE: 182-182 binding site SITE: 210-210 FOR-BINDING-OF: Chloride aspartate residue {D212} Ca+2-binding site SITE: 216-216 glutamate residue {E248} binding site SITE: 313-313 FOR-BINDING-OF: Chloride aspartate residue {D315} binding site SITE: 352-352 FOR-BINDING-OF: Chloride cysteine residue {C393} MODIFICATION: cysteine residue {C399} cysteine residue {C399} MODIFICATION: cysteine residue {C393} N-glycosylation site {N427} cysteine residue {C465} MODIFICATION: cysteine residue {C477} N-glycosylation site {N476} cysteine residue {C477} MODIFICATION: cysteine residue {C465}

Database Correlations

OMIM correlations PFAM correlations Entrez Gene 276 ENZYME 3.2.1.1