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replication protein A 32 kD subunit; RP-A p32; RP-A p34; replication factor A protein 2; RF-A protein 2 (RPA2 REPA2 RPA32 RPA34)

Function: - subunit of replication factor A - RPA2 is phosphorylated when cells traverse the G1->S boundary; phosphorylated through mitosis - RPA2 becomes hyperphosphorylated after ionizing radiation or UV radiation - phosphorylation of RPA2 by p34cdc2 (CDK1) may activate RPA activity & DNA replication; hyperphosphorylation may inactivate RPA, halt DNA replication & enhance DNA repair - phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci - can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku & DNA, & by CDC2 - binds to SERTAD3/RBT1 - interacts with TIPIN Compartment: - nucleus - also present in PML nuclear bodies - redistributes to discrete nuclear foci upon DNA damage Alternative splicing: named isoforms=3

General

phosphoprotein protein subunit

Properties

SIZE: entity length = 270 aa MW = 29 kD COMPARTMENT: cell nucleus MOTIF: serine-rich region {1-29} MOTIF: serine residue (SEVERAL) acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Thr phosphorylation site {T21} serine residue {23} Ser phosphorylation site {S29} Ser phosphorylation site {S33} lysine-rich region {37-45} MOTIF: lysine residue (SEVERAL) acidic region {95-123} MOTIF: acidic residue (SEVERAL) lysine-rich region {127-145} MOTIF: lysine residue (SEVERAL) TIPIN interaction {187-270} MOTIF: acidic region {247-270} MOTIF: acidic residue (SEVERAL)

Database Correlations

OMIM 179836 UniProt P15927 PFAM correlations Entrez Gene 6118 Kegg hsa:6118

References

  1. UniProt :accession P15927
  2. NIEHS-SNPs http://egp.gs.washington.edu/data/rpa2/
  3. Linn S How many pols does it take to replicate nuclear DNA? Cell 66:185 1991 PMID: 1855251
  4. Gottlieb TM, Jackson SP. Protein kinases and DNA damage. Trends Biochem Sci. 1994 Nov;19(11):500-3. Review. PMID: 7855895
  5. de Laat WL et al, Molecular mechonisms of nucleotide excision repair. Genes & Dev 13:768-85, 1999 PMID: 10197977

Component-of

replication factor-A (RFA/RPA)