RNA polymerase 1 L' (DNA-directed RNA polymerase 1 subunit RPA1, RNA polymerase 1 194 kD subunit, RPA194, POLR1A)

Function: - component of the RNA polymerase 1 complex - largest & catalytic core component of RNA polymerase 1 which synthesizes ribosomal RNA precursors - forms the polymerase active center together with the 2nd largest subunit - a single stranded DNA template strand of the promoter is positioned within the central active site cleft of pol 1 - a bridging helix emanates from POLR1A & crosses the cleft near the catalytic site & is thought to promote translocation of pol 1 by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (putative) nucleoside triphosphate + RNA(n) diphosphate + RNA(n+1) Structure: - - belongs to the RNA polymerase beta' chain family Compartment: nucleus, nucleolus (putative)

Properties

SIZE: entity length = 1717 aa MW = 194 kD COMPARTMENT: cell nucleus MOTIF: Zn finger C2H2-type SITE: 64-80 EFFECTOR-BOUND: Zn+2 MOTIF: Zn+2-binding site SITE: 64-64 Zn+2-binding site SITE: 67-67 Zn+2-binding site SITE: 74-74 Zn+2-binding site SITE: 77-77 Mg+2-binding site SITE: 588-588 Mg+2-binding site SITE: 590-590 Mg+2-binding site SITE: 592-592 Bridging helix {961-973}

Database Correlations

UniProt O95602 PFAM correlations ENZYME 2.7.7.6

References

UniProt :accession O95602