E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein (RNF144B, IBRDC2, P53RFP)

Function: - E3 ubiquitin-protein ligase - induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism - overexpression produces a decrease of ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death - protein modification; protein ubiquitination - auto-ubiquitinated - interacts with UBE2L3, UBE2L6 & LCMT2, as well as with BAX Structure: - the RING-type Zn⁺² finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (putative) - the transmembrane domain is essential for translocation to the mitochondria upon induction of apoptosis - belongs to the RBR family, RNF144 subfamily - contains 1 IBR-type Zn⁺² finger - contains 2 RING-type Zn⁺² fingers Compartment: - mitochondrial membrane - cytoplasm - mostly cytosololic - accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation Expression: - broadly expressed - highest expression in heart, ovary & testis - lowest expression in brain & thymus Note: - lacks the His in the RING-type domain 2 that is one of the conserved features of the family

General

membrane protein mitochondrial protein ring finger protein E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: entity length = 303 aa MW = 34 kD COMPARTMENT: mitochondria cytoplasm MOTIF: RING-finger {30-80} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Zinc finger NAME: Zinc finger SITE: 101-166 EFFECTOR-BOUND: Zn+2 RING-finger {193-222} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif transmembrane domain {258-278}

Database Correlations

UniProt Q7Z419 Pfam PF01485 Entrez Gene 255488 Kegg hsa:255488

References

UniProt :accession Q7Z419