E3 ubiquitin-protein ligase Arkadia (RING finger protein 111, RNF111)

Function: - role in the NODAL pathway of mesoderm patterning during embryonic development - acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs including SMAD7, induces their proteasomal degradation & thus enhances the transcriptional activity of TGF-beta & BMP. - ubiquitin conjugation, 3rd step - interacts with SMAD6, SMAD7, AXIN1 & AXIN2 - component of a complex containing RNF111, AXIN1 & SMAD7 Structure: contains 1 RING-type zinc finger Compartment: - nucleus, cytoplasm - upon TGF-beta treatment, translocates from nucleus to cytosol Alternative splicing: named isoforms=3 Expression: broadly expressed

General

ring finger protein E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: MW = 109 kD entity length = 994 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: interaction with AXIN1 {241-404} serine-rich region {252-464} MOTIF: serine residue (SEVERAL) histidine-rich region {494-523} MOTIF: histidine residue (SEVERAL) proline-rich region SITE: 629-781 MOTIF: proline residue (SEVERAL) RING-finger {942-983} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif

Database Correlations

OMIM 605840 UniProt Q6ZNA4 Pfam PF00097

References

UniProt :accession Q6ZNA4