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ribonuclease 3 (Drosha, p241, RNase3, RNASEN, RN3, RNASE3L)
Function:
1) executes initial step of microRNA (miRNA) processing in nucleus, cleavage of pri-miRNA to release pre-miRNA
2) role in pre-rRNA processing
3) cleaves double-strand RNA but does not cleave single-strand RNA
4) endonucleolytic cleavage to 5'-phosphomonoester.
5) interacts with Sp1
Compartment:
- nucleus
- a fraction is translocated to nucleolus during S phase of the cell cycle
Alternative splicing: named isoforms=3
Expression: ubiquitous
Structure:
- contains 1 DRBM domain (double-stranded RNA-binding domain) contains 2 RNase-3 domains
Related
eosinophil cationic protein (ribonuclease 3, RNase3, ECP)
short interfering double-stranded RNA; antisense oligonucleotide (siRNA, microRNA, miRNA)
General
endoribonuclease
Properties
SIZE: MW = 159 kD
entity length = 1374 aa
COMPARTMENT: cell nucleus
MOTIF: proline-rich region
SITE: 1-212
MOTIF: proline residue (SEVERAL)
arginine-rich region {219-316}
MOTIF: arginine residue (SEVERAL)
RNase 3 {876-1056}
RNase 3 {1107-1233}
DRBM {1260-1334}
Database Correlations
OMIM 608828
UniProt Q9NRR4
PFAM correlations
Entrez Gene 29102
ENZYME 3.1.26.3
References
- UniProt :accession Q9NRR4
- Protein Spotlight; Note: The dark side of RNA -
Issue 87 of October 2007
http://www.expasy.org/spotlight/back_issues/sptlt087.shtml
Component-of
microprocessor complex; pri-miRNA processing protein complex