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RNA polymerase 1
Function:
- synthesis of rRNA
- transcription of ribosomal RNA genes, present in tandem repeats
- synthesizes ribosomal RNA precursors
Structure:
- consists of at least 13 subunits
- pol 1 is composed of mobile elements & a core element with a central large cleft & probably a clamp element that moves to open & close the cleft (putative)
- a bridging helix emanates from POLR1A & crosses the cleft near the catalytic site & is thought to promote translocation of pol 1 by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (putative)
Compartment: nucleus, nucleolus
Interactions
molecular events
General
RNA polymerase
Properties
COMPARTMENT: nucleolus
MOTIF: active site
SUBUNITS: RNA polymerases 1 & 3 40 kD subunit
MOTIF: Tyr phosphorylation site {Y33}
RNA polymerase 1, 2, 3 17.1 kD subunit (RPABC3, RPB17, RPB8, hRPB8, POLR2H)
RNA polymerase I, 2, 2 7.0 kD subunit
MOTIF: Zn finger C4-type
SITE: 19-39
EFFECTOR-BOUND: Zn+2
RNA polymerase 1 L'
MOTIF: Zn finger C2H2-type
SITE: 64-80
EFFECTOR-BOUND: Zn+2
MOTIF: Zn+2-binding site
SITE: 64-64
Zn+2-binding site
SITE: 67-67
Zn+2-binding site
SITE: 74-74
Zn+2-binding site
SITE: 77-77
Mg+2-binding site
SITE: 588-588
Mg+2-binding site
SITE: 590-590
Mg+2-binding site
SITE: 592-592
Bridging helix {961-973}
RNA polymerase 1 L
MOTIF: Zn finger C4-type
SITE: 1070-1101
EFFECTOR-BOUND: Zn+2
RNA polymerases 1 & 3 subunit RPAC2
DNA-directed RNA polymerase 1 associated factor 53 kD subunit
DNA-directed RNA polymerase 1 subunit RPA43
MOTIF: Ser phosphorylation site {S60}
lysine-rich region {224-334}
MOTIF: lysine residue (SEVERAL)
Ser phosphorylation site {S316}
Ser phosphorylation site {S328}
DNA-directed RNA polymerase 1 subunit RPA12
MOTIF: Zn finger C4-type
SITE: 20-41
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 83-123
EFFECTOR-BOUND: Zn+2
Database Correlations
ENZYME 2.7.7.6
References
- Wikipedia: RNA polymerase
http://en.wikipedia.org/wiki/RNA_polymerase
- from NCBI bookshelf
The Cell - A Molecular Approach. 2nd ed.
Cooper, Geoffrey M.
Sunderland (MA): Sinauer Associates, Inc; c2000
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=RNA%20polymerase&rid=cooper.section.980
Component-of
macromolecular complex
Components
DNA-directed RNA polymerase 1 associated factor 53 kD subunit (PAF53)
DNA-directed RNA polymerase 1 subunit RPA12 (zinc ribbon domain-containing protein 1, ZNRD1, RPA12)
DNA-directed RNA polymerase 1 subunit RPA43 (twist neighbor protein, TWISTNB)
DNA-directed RNA polymerases I & 3 subunit RPAC2 (RNA polymerases 1 & 3 subunit AC2, DNA-directed RNA polymerase I subunit D, DNA-directed RNA polymerase 1 16 kD polypeptide, RPA16, RPC16, hRPA19, AC19, POLR1D)
RNA polymerase 1 L (RNA polymerase 1 135 kD subunit, RPA135, RNA polymerase 1 subunit 2, POLR1B)
RNA polymerase 1 L' (DNA-directed RNA polymerase 1 subunit RPA1, RNA polymerase 1 194 kD subunit, RPA194, POLR1A)
RNA polymerase 1, 2, 3 17.1 kD subunit (RPABC3, RPB17, RPB8, hRPB8, POLR2H)
RNA polymerase I, 2, 2 7.0 kD subunit
RNA polymerases 1 & 3 40 kD subunit; DNA-directed RNA polymerases 1 & 3 subunit RPAC1; RNA polymerases 1 & 3 subunit AC1; DNA-directed RNA polymerase I subunit C (RPA40, RPA39, RPC40, AC40, POLR1C, POLR1E)
RNA polymerases 1, 2, & 3 subunit RPABC1 (RNA polymerases 1, 2, & 3 subunit ABC1, RNA polymerase 2 23 kD subunit, RNA polymerase 2 subunit E, RNA polymerase 2 subunit 5, POLR2E, RPABC1, RPB25, RBP5, XAP4)
RNA polymerases 1, 2, & 3 subunit RPABC2 (RPB6, RNA polymerase 2 subunit F, POLR2F, POLRF, ABC2, RPABC14.4, RPB14.4, RPC15)