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restriction fragment length polymorphism (RFLP)

Clinical significance: - polymorphisms may arise from single base mutations, deletions or insertion of DNA - RFLP analysis is useful for genetic linkage studies to establish carrier (heterozygous) state in kindred of individuals known to be affected with specific genetic disorders Procedure: - analysis of DNA polymorphisms by agarose gel electrophoresis after cleavage of DNA with restriction endonucleases Turnaround time: 1-2 days

Related

restriction enzyme (endonuclease)

General

special chemistry test

References

  1. Medical Knowledge Self Assessment Program (MKSAP) 11, American College of Physicians, Philadelphia 1998
  2. Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed., W.B. Saunders, 1995