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Ras association domain-containing protein 1 (RASSF1, RDA32)
Function:
1) tumor suppressor (putative)
2) death receptor-dependent apoptosis
3) when associated with MOAP1, promotes BAX translocation to mitochondrial membranes in response to TNF & TNFSF10
4) inhibits proliferation
5) negatively regulates cell cycle progression G1/S-phase transition - regulates cyclin D1 protein
6) inhibits activation of STK3 & STK4
7) scaffold protein (putative)
9) interacts with XPAl, CDC20 (during prometaphase), STK3, STK4, NFRSF1A, TNFRSF10A, RSSF5, HRAS1, RSSF1, XPA
Compartment:
- isoform A localizes to microtubules during interphase, centrosomes associated with microtubules during prophase, spindle fibers & spindle poles at metaphase & anaphase, midzone during early telophase, midbody in late telophase in cells
- isoform C is predominantly nuclear
Alternative splicing: 7 alternate splice forms, A-G.
Expression:
- isoform A & isoform C are ubiquitously expressed
- isoform B is expressed in hematopoietic cells
Pathology:
- loss of RASSF1A seen in small cell lung cancer & some other cancer cell lines which correlate with hypermethylation of the CpG island promoter sequence of RASSF1A
Interactions
molecular events
General
Ras association domain-containing protein (RASSF)
zinc finger protein
Properties
SIZE: entity length = 344 aa
MW = 39 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: E4F1 interaction {1-119}
MOTIF: Ser phosphorylation site {S2}
Zinc finger
NAME: Zinc finger
SITE: 51-105
EFFECTOR-BOUND: Zn+2
Ser phosphorylation site {S188}
Ras-associating {198-292}
MOTIF: Thr phosphorylation site {T206}
SARAH {294-341}
MOTIF: binding site
SITE: 315-318
FOR-BINDING-OF: modulator of apoptosis 1
Database Correlations
OMIM 605082
UniProt Q9NS23
PFAM correlations
Entrez Gene 11186
Kegg hsa:11186
References
- UniProt :accession Q9NS23
- OMIM :accession 60508
- Atlas of Genetics & Cytogenetics in Oncology & Haematology
http://atlasgeneticsoncology.org/genes/RASSF1ID377.html