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Rab11 family-interacting protein 3; Rab11-FIP3; FIP3-Rab11; EF hands-containing Rab-interacting protein; eferin; arfophilin-1; MU-MB-17.148 (RAB11FIP3, ARFO1, KIAA0665)

Function: - acts as a regulator of endocytic traffic by participating in membrane delivery - required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis - also required for the structural integrity of the endosomal recycling compartment during interphase - may play a role in breast cancer cell motility by regulating actin cytoskeleton - forms a complex with Rab11 (RAB11A or RAB11B) & ARF6 - interacts with RAB11A; the interaction is direct - interacts with RAB11B, RAB25 & RAB11FIP4 - interacts with ARF6 - specifically interacts with ARF6 [3,4] - interacts with RACGAP1/MgcRacGAP; interaction takes place during late stage of cytokinesis & is required for recruitment to the midbody - interacts with ASAP1 & EXOC7 Structure: - homodimer - the RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B) - contains 2 EF-hand domains - contains 1 RBD-FIP domain Compartment: - recycling endosome membrane; peripheral membrane protein - cytoplasm, cytoskeleton, centrosome - in early mitosis, remains diffuse & distributed throughout cell - the onset of anaphase sequesters endosomes (veiscles) to the centrosomes at the opposite poles of the cell. - during telophase these vesicles move from the centrosomes, to the furrow, & then to the midbody to aid in abscission - interaction with Rab11 mediates localization to endosomes - interaction with ARF6 mediates localization to the midbody Alternative splicing: named isoforms=3

General

Ca+2 binding protein

Properties

SIZE: entity length = 756 aa MW = 82 kD COMPARTMENT: cytoplasm MOTIF: proline-rich region SITE: 5-197 MOTIF: proline residue (SEVERAL) serine-rich region {148-151} MOTIF: serine residue (SEVERAL) EF hand SITE: 202-237 MOTIF: Ca+2-binding site SITE: 215-226 EF hand SITE: 234-269 MOTIF: Ca+2-binding site SITE: 247-258 binding site SITE: 484-588 FOR-BINDING-OF: ARF subfamily protein coiled coil {485-694} RBD-FIP {716-756}

Database Correlations

OMIM 608738 UniProt O75154 Pfam PF09457 Entrez Gene 9727 Kegg hsa:9727

References

  1. UniProt :accession O75154
  2. Entrez Gene :accession 9727
  3. Fielding AB et al Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis. EMBO J. 2005 Oct 5;24(19):3389-99. Epub 2005 Sep 8. PMID: 16148947
  4. Shiba T et al Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1. Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15416-21. PMID: 17030804