apoptosis-associated speck-like protein containing a CARD; hASC; caspase recruitment domain-containing protein 5; PYD & CARD domain-containing protein; target of methylation-induced silencing 1 (PYCARD, ASC, CARD5, TMS1)

Function: - promotes caspase-mediated apoptosis - proapoptotic activity is mediated predominantly through the activation of caspase-9 - may be a component of the inflammasome - phosphorylated - forms complexes with other DAPIN domain-containing proteins - interacts with CIAS1/PYPAF1 & PYDC1 Structure: - interacts with CIAS1/PYPAF1 & PYDC1 via the DAPIN domain - contains 1 CARD domain - contains 1 DAPIN domain - only protein to contain both CARD & DAPIN domains Compartment: - cytoplasm - upstream of caspase activation, a redistribution from cytoplasm to aggregates occurs; these appear as hollow, perinuclear spherical, ball-like structures Alternative splicing: named isoforms=3 Expression: - widely expressed at low levels - detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon - low expression in placenta, liver & kidney - very low expression in skeletal muscle, heart & brain - detected in the leukemia cell lines HL-60 & U937 - not detected in Jurkat T-cell lymphoma or Daudi Burkitt's lymphoma - detected in the melanoma cell line WM35 - not detected in WM793 cells - not detected in HeLa cervical carcinoma cells & molt 4 lymphocytic leukemia cells Pathology: - in breast cancer, methylation-mediated silencing may affect genes & proteins that act as positive mediators of cell death

Related

apoptosis DNA methylation (promoter methylation) transcriptional silencing

Properties

SIZE: entity length = 195 aa MW = 22 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: DAPIN domain {1-91} CARD domain {107-195}

Database Correlations

OMIM 606838 UniProt Q9ULZ3 PFAM correlations Entrez Gene 29108 Kegg hsa:29108

References

UniProt :accession Q9ULZ3