pumilio homolog 2 (pumilio-2, PUM2, KIAA0235, PUMH2)

Function: 1) sequence-specific RNA-binding protein 2) regulates translation & mRNA stability by binding the 3' untranslated regions (UTRs) of mRNA targets 3) role in proliferation & self-renewal of stem cells by regulating translation of key transcripts (putative) 4) interacts with DAZ, DAZL & NANOS1 via its pumilio repeats 5) binds to RNA consensus sequence, related to Nanos Response Element (NRE), a 16 bp sequence found in the 3'UTR of the Drosophila hb mRNA 6) binds to the NRE Structure: - homodimer - contains 8 pumilio repeats - the pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut - the number as well as the specific sequence of the pumilio repeats determine the specificity for target mRNAs - contains 1 PUM-HD domain Compartment: cytoplasm Alternative splicing: named isoforms=3 Expression: - expressed in testis, ovary, stem cells, germ cells > brain, heart, kidney, liver, muscle, placenta, intestine, stomach - expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata, putamen. - expressed in all fetal tissues - dwn-regulated in keratinocytes upon UV B radiation

General

phosphoprotein RNA-binding protein

Properties

SIZE: MW = 114 kD entity length = 1066 aa COMPARTMENT: cytoplasm MOTIF: Ser phosphorylation site {S182} alanine-rich region {278-488} MOTIF: alanine residue (SEVERAL) glutamine-rich region {359-405} MOTIF: glutamine residue (SEVERAL) serine-rich region {520-687} MOTIF: serine residue (SEVERAL) PUM-HD {706-1048} MOTIF: Pumilio {726-761} Pumilio {762-797} Pumilio {798-835} Pumilio {836-871} Pumilio {872-907} Pumilio {908-943} Pumilio {944-979} Pumilio {983-1022}

Database Correlations

OMIM 607205 UniProt Q8TB72 Pfam PF00806

References

UniProt :accession Q8TB72