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twinfilin-1; protein A6; protein tyrosine kinase 9 (TWF1, PTK9)
Function:
- sequesters G-actin
- prevents actin filament assembly
- caps barbed ends of filaments
- regulates cell motility
- role in clathrin-mediated endocytosis & distribution of endocytic organelles (putative)
- phosphorylated on Ser & Thr
- interacts with capping protein
- interacts with TWF2 & PI(4,5)P2 (putative)
- when bound to PI(4,5)P2, it is down-regulated (putative)
Structure:
- belongs to the actin-binding proteins ADF family Twinfilin subfamily
- contains 2 ADF-H domains
Compartment:
- cytoplasm, cytoskeleton
- diffuse cytoplasmic localization
- perinuclear & G-actin-rich cortical actin structures sublocalization
- also found at membrane ruffles & cell-cell contacts (putative)
Alternative splicing: named isoforms=3
Expression:
- expressed in colon, testis, ovary, prostate & lung > brain, bladder & heart
- not detected in liver
Note:
- originally thought to have protein tyrosine kinase activity
General
phosphoprotein
Properties
SIZE: entity length = 350 aa
MW = 40 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: ADF-H 1 {2-139}
acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S143}
ADF-H 2 {175-313}
MOTIF: Tyr phosphorylation site {Y309}
Thr phosphorylation site {T349}
Database Correlations
OMIM 610932
UniProt Q12792
Pfam PF00241
Entrez Gene 5756
Kegg hsa:5756
References
- UniProt :accession Q12792
- Protein Spotlight; Note: molecular embrace -
Issue 73 of August 2006
http://web.expasy.org/spotlight/back_issues/sptlt073.shtml