protein kinase C-eta (PRKCH, PKCL, PRKCL)

Function: - Ca+2-independent, phospholipid-dependent - serine/threonine kinase - activated by diacylglycerol - 3 specific sites a) Thr-512 (activation loop of the kinase domain) b) Thr-655 (turn motif) c) Ser-674 (hydrophobic region) need to be phosphorylated for its full activation Structure: - C1 domain, containing the phorbol ester/DAG-type region 1 is the diacylglycerol sensor - C2 domain is a non-Ca+2 binding domain - belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily - contains 1 AGC-kinase C-terminal domain - contains 1 C2 domain - contains 2 phorbol-ester/DAG-type Zn+2 fingers - contains 1 protein kinase domain Expression: most abundant in lung, less in heart & skin Pathology: - defects in PRKCH may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain & resulting in loss of motor, sensory &/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic & environmental risk factors

Properties

SIZE: entity length = 682 aa MW = 78 kD COMPARTMENT: plasma membrane* CELL: most cell types* ORGANISM: eukaryote* STATE: active state MOTIF: C2 domain {12-112} MOTIF: binding site FOR-BINDING-OF: phospholipid Ca+2-binding site Zinc finger NAME: Zinc finger SITE: 170-221 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 244-294 EFFECTOR-BOUND: Zn+2 kinase domain SITE: 354-613 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 360-368 ATP-binding site NAME: ATP-binding site SITE: 383-383 aspartate residue {D478} Thr phosphorylation site {T512} AGC-kinase C-terminal {614-682} MOTIF: Thr phosphorylation site {T655} Ser phosphorylation site {S674}

Database Correlations

OMIM correlations MORBIDMAP 605437 UniProt P24723 PFAM correlations Entrez Gene 5583 KEGG correlations ENZYME 2.7.11.13

References

UniProt :accession P24723