protein kinase C (PKC)

A family of proteins Function: - in vivo, PKC prefers phosphorylation of Ser or Thr found close to a C-terminal basic residue - additional basic residues at the N- or C-terminal of the target amino acid enhances the Vmax & Km of phosphorylation Compartment: - apparently localize to focal adhesions [2]

Specific

protein kinase (C-mu protein kinase D1, PRKD1, PKD, PKD1, PRKCM) protein kinase C type-A protein kinase C type-B protein kinase C type-D2 (PRKD2, nPKC-D2, HSPC187) protein kinase C-nu (protein kinase D3, NPKC-nu, protein kinase EPK2, PRKD3, PRKCN)

General

allosteric enzyme serine/threonine kinase

Properties

SIZE: gel filtration = 87 kD COMPARTMENT: plasma membrane* CELL: most cell types* ORGANISM: eukaryote* STATE: active state MOTIF: DAG/PE-binding domain {N-TERMINAL} MOTIF: binding site EFFECTOR-BOUND: diacylglycerol Zinc finger NAME: Zinc finger (2) EFFECTOR-BOUND: Zn+2 C2 domain MOTIF: allosteric site EFFECTOR-BOUND: phosphatidylserine Ca+2-binding site EFFECTOR-BOUND: Ca+2 kinase domain MOTIF: ATP-binding site NAME: ATP-binding site MISC-INFO: DISTRIBUTION :REGIONAL BROAD :SUBCELLULAR PARTICULATE cytoplasm SUBSTRATE-SPECIFICITY :RANGE BROAD INHIBITOR = STAUROSPORINE INHIBITOR = QUERCITIN INHIBITOR = POLYMYXIN-B INHIBITOR = H-7 INHIBITOR = HIV_ENVELOPE_PROTEIN_GP120