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protein kinase A
Classification:
- 2 types: PKA1, PKA2
Function:
- phosphorylates a large number of substrates in cytoplasm & nucleus
- a number of inactive tetrameric holoenzymes are produced by combination of homo- or heterodimers of different regulatory subunits associated with two catalytic subunits
- cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to 4 cAMP & 2 free monomeric catalytic subunits
- activated by cAMP
Compartment:
- translocates into the nucleus (monomeric catalytic subunit)
- the inactive holoenzyme is found in the cytoplasm (putative)
Pharmacokinetics:
- protein kinase A has a long, but unspecified 1/2life
Interactions
molecular events
Related
glycogen storage disease type-X
Specific
protein kinase A1
protein kinase A2
General
allosteric enzyme
multisubunit protein
serine/threonine kinase
Properties
SIZE: MW = 170-190 kD
COMPARTMENT: cytoplasm
STATE: Inactive
SUBUNITS: PKA regulatory (2)
MOTIF: PEST region
cAMP-binding site (2)
EFFECTOR-BOUND: cyclic AMP
cAMP-binding site (2)
cAMP kinase (2)
MOTIF: S/T phosphorylation site
kinase domain
MOTIF: ATP-binding site
NAME: ATP-binding site
STATE: Inactive
MISC-INFO: DISTRIBUTION
:REGIONAL BROAD
:SUBCELLULAR PARTICULATE
cytoplasm
SUBSTRATE-SPECIFICITY
:RANGE BROAD
Ki [H-89] 48 PM
Database Correlations
ENZYME 2.7.1.37
References
- Armstrong DL
Calcium channel regulation by calcineurin, a Ca+2-activated
phosphatase in mammalian brain
TINS 12(3):117 1989
PMID: 2469218
- Rogers S et al,
Amino acids common to rapidly degraded proteins: The PEST
hypothesis
Science 234:364 1986 (1/2 life)
PMID: 2876518
- UniProt :accession P17612
Components
cAMP-dependent protein kinase catalytic subunit or protein kinase A [PKA] catalytic subunit
protein kinase A [PKA] regulatory subunit