protein arginine N-methyltransferase 8; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 (PRMT8, HRMT1L4)

Function: - membrane-associated protein arginine N-methyltransferase - catalyzes formation of omega-N monomethylarginine (MMA) & asymmetrical dimethylarginine (aDMA) - able to mono- & dimethylate EWS protein; - role toward EWS remains unclear as it still interacts with fully methylated EWS - homodimers & heterodimers with PRMT1 or PRMT2, recruiting PRMT1 to the cell membrane - interacts with PRMT2 & FYN (via the SH3 domain) - interacts with EWS; independently of EWS methylation status Kinetic parameters: - KM=1.3 uM for GRGGFGGRGGFRGGRGG-NH2 Structure: - the SH3-binding motifs mediate the interaction with SH3 domain-containing proteins such as PRMT2 & FYN, possibly leading to displace the N-terminal domain & activate the protein the N-terminal region (1-60) inhibits the enzymatic activity - belongs to the protein arginine N-methyltransferase family, PRMT8 subfamily Compartment: - cell membrane; lipid-anchor; cytoplasmic side Alternative splicing: named isoforms=2 Expression: brain-specific

General

myristoylated protein protein arginine N-methyltransferase (PRMT)

Properties

SIZE: entity length = 394 aa MW = 45 kD COMPARTMENT: cytoplasm MOTIF: myristoylation site SITE: G2 EFFECTOR-BOUND: myristate SH3-binding site NAME: SH3-binding site SITE: 29-42 SH3-binding site NAME: SH3-binding site SITE: 53-58 binding site SITE: 86-86 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 95-95 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 119-119 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 141-141 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 170-170 FOR-BINDING-OF: S-adenosylmethionine

Database Correlations

OMIM 610086 UniProt Q9NR22 Pfam PF06325 Entrez Gene 56341 Kegg hsa:56341

References

UniProt :accession Q9NR22