Search
protein kinase C-epsilon (PKC-N, PRKCE)
Function:
- Ca+2-independent, phospholipid-dependent
- serine/hreonine kinase
- activated by diacylglycerol
- forms a ternary complex with TRIM63 & GN2BL1
- 3 specific sites
a) Thr-566 (activation loop of the kinase domain)
b) Thr-710 (turn motif)
c) Ser-729 (hydrophobic region) need to be phosphorylated for its full activation
- phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729
- PDGF activates PKC epsilon [2]
Structure:
- C1 domain, containing the phorbol ester/DAG-type region 1 is the diacylglycerol sensor
- C2 domain is a non-Ca+2 binding domain
- belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
- contains 1 AGC-kinase C-terminal domain
- contains 1 C2 domain
- contains 2 phorbol-ester/DAG-type Zn+2 fingers
- contains 1 protein kinase domain
General
protein kinase C type-B
Properties
SIZE: entity length = 737 aa
MW = 84 kD
COMPARTMENT: plasma membrane*
CELL: most cell types*
ORGANISM: eukaryote*
STATE: active state
MOTIF: C2 domain {1-99}
MOTIF: binding site
FOR-BINDING-OF: phospholipid
Ca+2-binding site
Zinc finger
NAME: Zinc finger
SITE: 169-220
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 242-292
EFFECTOR-BOUND: Zn+2
Ser phosphorylation site {S337}
Thr phosphorylation site {T349}
kinase domain
SITE: 408-668
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 414-422
ATP-binding site
NAME: ATP-binding site
SITE: 437-437
aspartate residue {D532}
Thr phosphorylation site {T566}
AGC-kinase C-terminal {669-737}
MOTIF: Thr phosphorylation site {T703}
Thr phosphorylation site {T710}
Ser phosphorylation site {S729}
Database Correlations
OMIM 176975
UniProt Q02156
PFAM correlations
Entrez Gene 5581
KEGG correlations
ENZYME 2.7.11.13
References
- UniProt :accession Q02156
- Moriya S, Kazlauskas A, Akimoto K, Hirai S, Mizuno K,
Takenawa T, Fukui Y, Watanabe Y, Ozaki S, Ohno S.
Platelet-derived growth factor activates protein kinase C
epsilon through redundant and independent signaling pathways
involving phospholipase C gamma or phosphatidylinositol
3-kinase.
Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):151-5.
PMID: 8552594