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protein kinase C-alpha (PKC-III, PRKCA, PKCA PRKACA)
Function:
- Ca+2-activated, phospholipid-dependent serine/threonine kinase
- may play a role in cell motility by phosphorylating CSPG4
- activated by diacylglycerol
- phosphorylates a range of cellular proteins
- interacts with CENTA1, CSPG4 & PRKCABP
- binds to SDPR in the presence of phosphatidylserine
Cofactor:
- binds 3 Ca+2 per subunit
- Ca+2 bound to the C2 domain (putative)
Structure:
- belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
- contains 1 AGC-kinase C-terminal domain
- contains 1 C2 domain
- contains 2 phorbol-ester/DAG-type Zn+2 fingers
- contains 1 protein kinase domain
Interactions
molecular events
General
protein kinase C type-A
zinc finger protein
Properties
SIZE: entity length = 672 aa
MW = 77 kD
COMPARTMENT: plasma membrane*
CELL: most cell types*
ORGANISM: eukaryote*
MOTIF: Zinc finger
NAME: Zinc finger
SITE: 36-86
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 101-151
EFFECTOR-BOUND: Zn+2
C2 domain {172-260}
MOTIF: binding site
FOR-BINDING-OF: phospholipid
Ca+2-binding site
SITE: 186-186
Ca+2-binding site
SITE: 187-187
Ca+2-binding site
SITE: 193-193
Ser phosphorylation site {S226}
Ca+2-binding site
SITE: 246-246
Ca+2-binding site
SITE: 247-247
Ca+2-binding site
SITE: 248-248
Ca+2-binding site
SITE: 252-252
Ca+2-binding site
SITE: 254-254
Ser phosphorylation site {S319}
kinase domain
SITE: 339-597
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 345-353
ATP-binding site
NAME: ATP-binding site
SITE: 368-368
aspartate residue {D463}
Thr phosphorylation site {T494}
Thr phosphorylation site {T495}
Thr phosphorylation site {T497}
Thr phosphorylation site {T501}
AGC-kinase C-terminal {598-668}
MOTIF: Thr phosphorylation site {T631}
Thr phosphorylation site {T638}
Ser phosphorylation site {S657}
Tyr phosphorylation site {Y658}
STATE: active state
MISC-INFO: RECOGNITION-MOTIF
:SEQUENCE XRXX[S*]XRX
INHIBITOR = SPHINGOSINE
Database Correlations
OMIM 176960
UniProt P17252
PFAM correlations
Entrez Gene 5578
Kegg hsa:5578
ENZYME 2.7.11.13
References
- Kemp BE, Pearson RB.
Protein kinase recognition sequence motifs.
Trends Biochem Sci. 1990 Sep;15(9):342-6. Review.
PMID: 2238044
- UniProt :accession P17252