pinin (140 kD nuclear & cell adhesion-related phosphoprotein, domain-rich serine protein, DRS-protein, DRSP, melanoma metastasis clone A protein, desmosome-associated protein, SR-like protein, nuclear protein SDK3, PNN, DRS, MEMA)

Function: 1) transcriptional activator 2) binds to E-box 1 core sequence 5'CAGGTG-3' of the E-cadherin promoter gene 3) reverses CTBP1-mediated transcription repression. 4) regulation of alternative pre-mRNA splicing. 5) associates to spliced mRNA within 60 nucleotides upstream of the 5' splice sites 6) establishment & maintenance of epithelia cell-cell adhesion 7) forms complex with SR proteins 8) found in a mRNP complex with RNPS1 9) interacts with C6orf111/SRRP130, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 & SRRM2 10) component of spliceosome C complex Compartment: - nucleus, nuclear speckle - cell junction, cell membrane, - desmosome of intercellular adherens junction (cell-cell contact) - not a nucleocytoplasmic shuttling protein Alternative splicing: named isoforms=2 Expression: - expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain, smooth muscle, skeletal muscle Pathology: 1) tumor suppressor for renal cell carcinoma (putative) 2) expressed strongly in melanoma metastasis lesions & advanced primary tumors

Properties

SIZE: MW = 81 kD entity length = 716 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: binding site SITE: 1-283 FOR-BINDING-OF: RNA-binding protein with serine-rich domain 1 MOTIF: 5' splicing {1-166} binding site SITE: 1-97 FOR-BINDING-OF: keratin, type II cytoskeletal 8 keratin, type I cytoskeletal 18 keratin, type I cytoskeletal 19 coiled coil {1-31} acetylation site SITE: 1-1 EFFECTOR-BOUND: acetyl Ser phosphorylation site {S65} coiled coil {162-233} glutamate-rich region {171-470} MOTIF: glutamate residue (SEVERAL) coiled coil {286-378} Ser phosphorylation site {S346} Ser phosphorylation site {S442} coiled coil {445-466} glutamine-rich region {468-519} MOTIF: glutamine residue (SEVERAL) serine-rich region {551-703} MOTIF: serine residue (SEVERAL) binding site SITE: 605-716 FOR-BINDING-OF: cyclophilin G MOTIF: arginine-rich region {631-716} MOTIF: arginine residue (SEVERAL)

Database Correlations

OMIM 603154 UniProt Q9H307 PFAM correlations

References

UniProt :accession Q9H307