Search
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1; phospholipase C-zeta-1; PLC-zeta-1; testis-development protein NYD-SP27 (PLCZ1)
Function:
- in vitro, hydrolyzes Ptdins(4,5)P2 (Ca+2-dependent)
- triggers intracellular Ca+2 oscillations in oocytes solely during M phase
- involved in inducing oocyte activation & initiating embryonic development up to the blastocyst stage
- candidate egg-activating soluble sperm factor transferred from sperm into the egg cytoplasm following gamete membrane fusion
- may exert an inhibitory effect on phospholipase-C-coupled processes that depend on Ca+2 & protein kinase C, including CFTR trafficking & function
- interacts via its C2 domain with Ptdins(3)P &, to a lesser extent, Ptdins(5)P in vitro (putative)
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Cofactor: Ca+2 (putative)
Structure:
- the EF-hand & C2 domains are essential for triggering Ca+2 oscillating activity & the regulation of PLCZ1 enzyme activity (putative)
- the X-Y linker region between PI-PLC X-box & Y-box domains may be a target for proteolysis & may play an important regulatory role during fertilization (putative)
- contains 1 C2 domain
- contains 1 EF-hand domain
- contains 1 PI-PLC X-box domain
- contains 1 PI-PLC Y-box domain
Compartment:
- nucleus (putative), cytoplasm, perinuclear region (putative)
- exhibits alternative cytoplasmic/nuclear localization during development
- translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis & localizes again to the pronucleus at interphase following meiosis & mitosis (putative)
Alternative splicing: named isoforms=3
Expression:
- expressed specifically in testis
- weakly expressed in pancreatic-duct cells
Pathology:
- up-regulated in pancreatic-duct cells from patients with cystic fibrosis
General
Ca+2 binding protein
nuclear protein
phosphodiesterase
Properties
SIZE: entity length = 608 aa
MW = 70 kD
COMPARTMENT: cytoplasm
nuclear membrane
MOTIF: EF hand
SITE: 35-70
PI-PLC X-box {155-299}
MOTIF: histidine residue {H170}
histidine residue {H215}
PI-PLC Y-box {349-465}
C2 domain {469-572}
MOTIF: binding site
FOR-BINDING-OF: phospholipid
Ca+2-binding site
Database Correlations
OMIM 608075
UniProt Q86YW0
PFAM correlations
Kegg hsa:8986
ENZYME 3.1.4.11
References
UniProt :accession Q86YW0