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protein kinase C-delta (PRKCD)
Function:
- Ca+2-independent, phospholipid-dependent
- serine/threonine kinase
- activated by diacylglycero
- phosphorylates MUC1 in the C-terminal & regulates the interaction between MUC1 & beta-catenin
- interacts with PDK1, RAD9A, CDCP1 & MUC1
- 3 specific sites;
a) Thr-507 (activation loop of the kinase domain)
b) Ser-645 (turn motif)
c) Ser-664 (hydrophobic region) need to be phosphorylated for its full activation
- autophosphorylated &/or phosphorylated
- Thr-507 phosphorylatio not a prerequisite for enzymatic activity
Structure:
- C1 domain, containing the phorbol ester/DAG-type region is the diacylglycerol sensor
- C2 domain is a non-Ca+2 binding domain; binds proteins containing phosphotyrosine in a sequence-specific manner
- belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
- contains 1 AGC-kinase C-terminal domain
- contains 1 C2 domain
- contains 2 phorbol-ester/DAG-type Zn+2 fingers
- contains 1 protein kinase domain
Compartment: cytoplasm, peripheral membrane (putative)
General
protein kinase C type-B
Properties
SIZE: entity length = 676 aa
MW = 77 kD
COMPARTMENT: cytoplasm
STATE: active state
MOTIF: C2 domain {1-90}
MOTIF: binding site
FOR-BINDING-OF: phospholipid
Ca+2-binding site
lysine residue {48}
histidine residue {62}
arginine residue {67}
glutamate residue {123}
Zinc finger
NAME: Zinc finger
SITE: 158-208
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 230-280
EFFECTOR-BOUND: Zn+2
Tyr phosphorylation site {Y313}
kinase domain
SITE: 349-603
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 355-363
ATP-binding site
NAME: ATP-binding site
SITE: 378-378
aspartate residue {D473}
Thr phosphorylation site {T507}
AGC-kinase C-terminal {604-675}
MOTIF: Ser phosphorylation site {S645}
Ser phosphorylation site {S664}
Database Correlations
OMIM 176977
UniProt Q05655
PFAM correlations
Entrez Gene 5580
KEGG correlations
ENZYME 2.7.11.13
References
UniProt :accession Q05655