Search
protein kinase C-beta 1 (PKC-IIa, PRKCB1)
Function:
1) Ca+2-activated, phospholipid-dependent, Ser- & Thr- specific enzyme
2) component of the NF-kappa-B signaling axis responsible for survival & activation of B-cells after BCR cross-linking
3) phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate; subsequent autophosphorylation of Thr-642 maintains catalytic competence, & autophosphorylation on Ser-661 appears to release the kinase into the cytosol
4) similarly, isoform beta-II is autophosphorylated on Thr-640 & Ser-659, subsequent to phosphorylation on Thr-500
5) autophosphorylated on other sites i.e in the N-terminal & hinge regions have no effect on PKC activity
6) interacts with PDK1, PRKCBP1
Cofactor: binds 3 Ca+2 per subunit; binds via C2 domain
Compartment: cytoplasm, peripheral membrane
Alternative splicing:
Properties
GENERAL: protein kinase C-beta
SIZE: entity length = 671 aa
MW = 77 kD
COMPARTMENT: cytoplasm
FORM: protein kinase C-beta 1
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S16}
Thr phosphorylation site {T17}
Zinc finger
NAME: Zinc finger
SITE: 36-86
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 101-151
EFFECTOR-BOUND: Zn+2
C2 domain {173-260}
MOTIF: binding site
FOR-BINDING-OF: phospholipid
Ca+2-binding site
SITE: 186-186
Ca+2-binding site
SITE: 187-187
Ca+2-binding site
SITE: 193-193
Tyr phosphorylation site {Y195}
Ca+2-binding site
SITE: 246-246
Ca+2-binding site
SITE: 247-247
Ca+2-binding site
SITE: 248-248
Ca+2-binding site
SITE: 251-251
Ca+2-binding site
SITE: 252-252
Ca+2-binding site
SITE: 254-254
Thr phosphorylation site {T314}
Thr phosphorylation site {T324}
kinase domain
SITE: 342-600
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 348-356
ATP-binding site
NAME: ATP-binding site
SITE: 371-371
aspartate residue {D466}
Thr phosphorylation site {T500}
Thr phosphorylation site {T504}
AGC-kinase C-terminal {601-671}
MOTIF: Thr phosphorylation site {T635}
Thr phosphorylation site {T642}
Ser phosphorylation site {S661}
STATE: active state
MISC-INFO: RECOGNITION-MOTIF
:SEQUENCE XRXX[S*]XRX
:REF " - Kemp BE, Pearson RB.
Protein kinase recognition sequence motifs.
Trends Biochem Sci. 1990 Sep;15(9):342-6. Review.
PMID: 2238044"
INHIBITOR = SPHINGOSINE
Database Correlations
OMIM 176970
UniProt P05771
PFAM correlations
Entrez Gene 5579
Kegg hsa:5579
ENZYME 2.7.11.13
References
UniProt :accession P05771