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pim1 proto-oncogene protein

Function: - Pim-1 interacts with & may play a role in promoting complex formation between NuMA, HP1-beta, dynein & dynactin which is necessary for mitosis - during prometaphase, pim-1 interacts with NuMA & centromere associated protein HP1 - Pim-1 phosphorylates NuMA [4]. - Hsp90-alpha & hsp90-beta interact with pim-1 & affect its stablization & function. - Pim-1 phosphorylation of cdc25A upregulates phosphatase activity - Pim-1 is regulated by various cytokines including GM-CSF, G-CSF, interleukin-3, interferon-alpha & interleukin-6 Compartment: - during the cell cycle, pim-1 moves from the nucleus & cytoplasm in interphase to the spindle poles during mitosis - in late metaphase/early anaphase pim-1 associates with the spindle & moves to the cleavage furrow during telophase Pathology: - overexpressed in a subset of hematolymphoid malignancies - also expressed in high levels in malignant prostate tissue, but absent in metastatic tissue Note: - there is a pim1 gene in Saccharomyces pombe that is a homolog of RCC1 - the product of the yeast gene is a serine/threonine kinase implicated in coupling S -> M phase transition in the cell cycle

Interactions

molecular events

Related

pim-1 proto-oncogene

General

proto oncogene protein serine/threonine kinase

Properties

SIZE: MW = 34-36 kD entity length = 313 aa COMPARTMENT: cytoplasm MOTIF: S/T phosphorylation site kinase domain MOTIF: ATP-binding site NAME: ATP-binding site STATE: active state MISC-INFO: RECOGNITION-MOTIF :SEQUENCE KRRA[S*]GP :REF "Friedmann et al Arch Biochem Biophys 298:594 1992"

Database Correlations

OMIM 164960 UniProt P11309 Entrez Gene 5292

References

  1. te Riele H, Maandag ER, Clarke A, Hooper M, Berns A. Consecutive inactivation of both alleles of the pim-1 proto-oncogene by homologous recombination in embryonic stem cells. Nature 348:649 1990 PMID: 2250720
  2. Padma R & Nagarajan L The human PIM-1 gene product is a protein serine kinase. Cancer Res 51:2486 1991 PMID: 1826633
  3. Journal Watch 21(20):162, 2001 Dhanasekaran SM et al Delineation of prognostic biomarkers in prostate cancer. Nature 412:822, 2001 PMID: 11518967
  4. Bhattacharya N et al. Pim-1 associates with protein complexes necessary for mitosis. Chromosoma 111:80-95, 2002 PMID: 12111331
  5. Mizuno K et al. Regulation of Pim-1 by Hsp90. Biochemical & Biophysical Research Communications 281:663-669, 2001 PMID: 11237709