partitioning-defective 3 homolog (PARD-3 ,PAR-3, atypical PKC isotype-specific-interacting protein, ASIP, CTCL tumor antigen se2-5, PAR3-alpha, PARD3, PAR3, PAR3A)

Function: 1) adapter protein involved in asymmetrical cell division & cell polarization processes 2) role in formation of epithelial tight junctions 3) association with PARD6B inhibits interaction with F11R/JAM1, thus preventing tight junction assembly (putative) 4) PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins 5) interacts with PARD6A & PARD6B 6) isoform 2, but not at least isoform 3 interacts with PRKCZ 7) interacts with PRCKI 8) component of complex with PARD6A or PARD6B, PRKCI or PRKCZ & CDC42 or RAC1 9) interacts with F11R/JAM1 10) phosphorylated by PRKCZ Structure: - belongs to the PAR3 family. - contains 3 PDZ (DHR) domains Compartment: - intracytoplasmic membrane, cell junction, cell membrane - partially localized along the cell-cell contact region - colocalizes with PARD6A & PRKCI at epithelial tight junctions Alternative splicing: named isoforms=10 Expression: widely expressed Pathology: - autoantibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphoma

Properties

SIZE: MW = 151 kD entity length = 1356 aa COMPARTMENT: cytoplasm MOTIF: PDZ domain NAME: PDZ domain SITE: 271-359 Ser phosphorylation site {S383} PDZ domain NAME: PDZ domain SITE: 461-546 PDZ domain NAME: PDZ domain SITE: 590-677 PRKCZ interaction {712-936} MOTIF: Ser phosphorylation site {S827} lysine-rich region {984-1042} MOTIF: lysine residue (SEVERAL) coiled coil {1049-1077} coiled coil {1151-1174} coiled coil {1201-1224} coiled coil {1280-1301}

Database Correlations

OMIM 606745 UniProt Q8TEW0 Pfam PF00595

References

UniProt :accession Q8TEW0