mitogen-activated protein kinase 12; extracellular signal-regulated kinase 6; ERK-6; ERK5; stress-activated protein kinase 3; mitogen-activated protein kinase p38 gamma; MAP kinase p38 gamma (MAPK12, ERK6, SAPK3)

Function: - responds to activation by environmental stress & pro-inflammatory cytokines by phosphorylating downstream targets - role in myoblast differentiation - role in down-regulation of cyclin D1 in response to hypoxia in adrenal cells - MAPK12 may inhibit cell proliferation while promoting differentiation - phosphorylates myelin-basic protein - activated by phosphorylation on Thr & Tyr (putative) - dually phosphorylated on Thr-183 & Tyr-185, which activates the enzyme (putative) - interacts with the PDZ domain of syntrophin SNTA1 - interacts with SH3BP5 Cofactor: binds 2 Mg⁺² Structure: - monomer - the TXY motif contains the Thr & Tyr whose phosphorylation activates the MAP kinases - belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily - contains 1 protein kinase domain Compartment: - cytoplasm. mitochondrion - mitochondrial when associated with SH3BP5 Expression: highly expressed in skeletal muscle & heart

Properties

SIZE: entity length = 367 aa MW = 42 kD COMPARTMENT: mitochondria cytoplasm STATE: active state MOTIF: kinase domain SITE: 27-311 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 33-41 Tyr phosphorylation site {Y27} ATP-binding site NAME: ATP-binding site SITE: 56-56 aspartate residue {D153} TXY {183-185} Thr phosphorylation site {T183} Tyr phosphorylation site {Y185}

References

UniProt :accession P53778

Databases & Figures

OMIM 602399 UniProt P53778 Pfam PF00069 Entrez Gene 6300 Kegg hsa:6300 ENZYME 2.7.11.24 MAP kinase pathways