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transcription initiation factor TFIID subunit 1; cell cycle gene 1 protein; TBP-associated factor 250 kD; p250; transcription initiation factor TFIID 250 kD subunit; TAF(II)250; TAFII-250; TAFII250 (TAF1, BA2R, CCG,1 CCGS, TAF2A)

Function: - largest component & core scaffold of the TFIID basal transcription factor complex - binds all other subunits of TFIID - role in progression through the late G1 phase of the cell cycle - contains novel N- & C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors - phosphorylates TP53 on Thr-55 destabilizing p53 leading to MDM2-mediated degradation of TP53 & inducing G1 progression [3] - essential for progression of the G1 phase of the cell cycle - phosphorylates GTF2A1 & GTF2F1 on Ser - possesses DNA- binding activity - TAF1, when part of the TFIID complex, interacts with C-terminus of TP53 - RB1 interacts with the N-terminal domain of TAF1 - inhibited by RB1 - interacts with ASF1A & ASF1B - autophosphorylates on Ser - phosphorylated by casein kinase 2 in vitro Cofactor: Mg+2 Structure: - belongs to the TAF1 family - contains 2 bromo domains - contains 1 HMG box DNA-binding domain - contains 2 protein kinase domains Compartment: nucleus Alternative splicing: named isoforms=4 Pathology: - defects in TAF1 are associated with X-linked torsion dystonia type 3

Interactions

molecular events

Related

cell cycle G1 gene

General

DNA-binding protein serine/threonine kinase transcription factor IID subunit

Properties

SIZE: entity length = 1872 aa MW = 213 kD COMPARTMENT: cell nucleus STATE: active state MOTIF: kinase domain SITE: 1-414 MOTIF: ATP-binding site NAME: ATP-binding site Ser phosphorylation site {S137} proline-rich region SITE: 157-165 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S307} HMG box SITE: 1195-1273 ASF1A/ASF1B {1342-1629} MOTIF: nuclear translocation signal {1351-1358} cysteine residue {C1364} MODIFICATION: cysteine residue {C1619} bromodomain {1397-1467} kinase domain SITE: 1425-1872 MOTIF: ATP-binding site NAME: ATP-binding site bromodomain {1520-1590} cysteine residue {C1619} MODIFICATION: cysteine residue {C1364} acidic region {1627-1872} MOTIF: acidic residue (SEVERAL) Ser phosphorylation site {S1826}

Database Correlations

OMIM correlations UniProt P21675 PFAM correlations Entrez Gene 6872 Kegg hsa:6872 ENZYME 2.7.11.1

References

  1. Ruppert S et al Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature 362:175 1993 PMID: 7680771
  2. Hisatake K et al The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1. Nature 362:179 1993 PMID: 8450888
  3. Li HH et al Phosphorylation of Thr-55 by TAF1 mediates degradation of p53, a role for TAF1 in cell G1 progression. Molecular Cell 13:867-878, 2004 PMID: 15053879
  4. UniProt :accession P21675
  5. NIEHS-SNPs http://egp.gs.washington.edu/data/taf1/

Component-of

transcription factor IID (TFIID)