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8-oxoguanine DNA glycosylase; N-glycosylase/DNA lyase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase (OGG1, MMH, MUTM, OGH1, FAPY)
Function:
- DNA repair enzyme that incises DNA at 8-oxoG residues
- excises 7,8-dihydro-8-oxoguanine & 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA
- as a beta-lyase activity that nicks DNA 3' to the lesion
- the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar & a product with a terminal 5'-phosphate
Structure: belongs to the type-1 OGG1 family
Compartment:
- isoform 1A: nucleus
- isoform 2A: mitochondrion
Alternative splicing: named isoforms=8
Expression: ubiquitous
Pathology:
- defects in OGG1 are associated with tumor formation
- defects in OGG1 are a cause of renal cell carcinoma
General
DNA glycosylase
Properties
SIZE: entity length = 345 aa
MW = 39 kD
COMPARTMENT: mitochondria
cell nucleus
MOTIF: DNA-binding motif
SITE: 149-149
DNA-binding motif
SITE: 154-154
DNA-binding motif
SITE: 204-204
lysine residue {K249}
binding site
SITE: 266-266
FOR-BINDING-OF: 7,8-dihydro-8-oxo-guanine
binding site
SITE: 268-268
FOR-BINDING-OF: 7,8-dihydro-8-oxo-guanine
DNA-binding motif
SITE: 270-270
DNA-binding motif
SITE: 287-287
binding site
SITE: 315-315
FOR-BINDING-OF: 7,8-dihydro-8-oxo-guanine
binding site
SITE: 319-319
FOR-BINDING-OF: 7,8-dihydro-8-oxo-guanine
Database Correlations
OMIM correlations
MORBIDMAP 601982
UniProt O15527
PFAM correlations
Entrez Gene 4968
Kegg hsa:4968
ENZYME 4.2.99.18
References
- UniProt :accession O15527
- NIEHS-SNPs
http://egp.gs.washington.edu/data/ogg1/
- Seeberg E et al
The base excision repair pathway.
TIBS 20:391 1995
PMID: 8533150