NuA4 histone acetyltransferase (HAT) complex

Function: - role in transcriptional activation of certain genes via acetylation of nucleosomal histone H4 & H2A - acetylation alters nucleosome - DNA interactions & positively regulates transcription - ATPase & helicase activities contributed by association of RUVBL1 & RUVBL2 with EP400 - NuA4 complex interacts with MYC & the adenovirus E1A protein - may be involved in: a) oncogene & proto-oncogene mediated growth induction b) tumor suppressor mediated growth arrest c) replicative senescence d) apoptosis e) DNA repair Structure: (subunits) - EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 & VPS72/YL1 - HTATIP/TIP60 is the catalytic subunit

Properties

COMPARTMENT: cell nucleus SUBUNITS: histone acetyltransferase KAT5 COMPARTMENT: cytoplasm cell nucleus MOTIF: Ser phosphorylation site {S86} Ser phosphorylation site {S90} Zn finger C2HC-type SITE: 261-283 EFFECTOR-BOUND: Zn+2 lysine residue {K327} cysteine residue {C369} binding site SITE: 372-372 FOR-BINDING-OF: Acetyl-CoA Acetyl-CoA binding {377-383} binding site SITE: 407-407 FOR-BINDING-OF: Acetyl-CoA E1A-binding protein p400 COMPARTMENT: cell nucleus MOTIF: glutamine-rich region {278-281} MOTIF: glutamine residue (SEVERAL) Thr phosphorylation site {T314} Ser phosphorylation site {S315} proline-rich region SITE: 316-319 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T320} Ser phosphorylation site {S321} glutamate-rich region {426-437} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S736} Ser phosphorylation site {S755} HSA {799-871} Ser phosphorylation site {S941} Ser phosphorylation site {S943} Thr phosphorylation site {T945} Interactions with RUVBL1 and RUVBL2 {951-1365} MOTIF: helicase NAME: helicase SITE: 1103-1268 ATP-binding site NAME: ATP-binding site SITE: 1116-1123 DEAD/H box NAME: DEAD/H box SITE: 1219-1222 alanine-rich region {1517-1522} MOTIF: alanine residue (SEVERAL) Ser phosphorylation site {S1547} Ser phosphorylation site {S1728} Ser phosphorylation site {S1732} Helicase C-terminal {1899-2056} Myb-like {2360-2429} ZNF42 interaction {2524-2789} MOTIF: proline-rich region SITE: 2543-2554 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 2557-2564 MOTIF: proline residue (SEVERAL) glutamine-rich region {2756-2784} MOTIF: glutamine residue (SEVERAL) Thr phosphorylation site {T2813} proline-rich region SITE: 2828-2834 MOTIF: proline residue (SEVERAL) transformation/transcription domain-associated protein MOTIF: proline-rich region SITE: 485-526 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S1628} TP53 interaction {2010-2388} MOTIF: nuclear translocation signal {2047-2062} Ser phosphorylation site {S2051} Ser phosphorylation site {S2077} Thr phosphorylation site {T2333} Thr phosphorylation site {T2601} FAT domain NAME: FAT domain SITE: 2704-3275 MOTIF: Tyr phosphorylation site binding site FOR-BINDING-OF: Paxillin PI3K/PI4K {3528-3826} FATC {3827-3859} bromodomain-containing protein 8 COMPARTMENT: cell nucleus MOTIF: coiled coil {97-171} Ser phosphorylation site {S637} Ser phosphorylation site {S641} bromodomain {724-794} bromodomain {1120-1190} MOTIF: Thr phosphorylation site {T1151} Ser phosphorylation site {S1152} enhancer of polycomb homolog 1 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S357} DNA methyltransferase 1-associated protein 1 COMPARTMENT: cell nucleus MOTIF: SANT {149-199} coiled coil {225-275} Thr phosphorylation site {T445} Ser phosphorylation site {S448} serine-rich region {454-460} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S456} RuvB-like 1 COMPARTMENT: cell nucleus MOTIF: ATP-binding site NAME: ATP-binding site SITE: 70-77 RuvB-like 2 COMPARTMENT: cytoplasm cell nucleus MOTIF: ATP-binding site NAME: ATP-binding site SITE: 77-84 Ser phosphorylation site {S220} inhibitor of growth protein 3 COMPARTMENT: cell nucleus MOTIF: serine-rich region {313-329} MOTIF: serine residue (SEVERAL) Zn finger PHD-type NAME: Zn finger PHD-type SITE: 360-409 EFFECTOR-BOUND: Zn+2 actin MOTIF: coiled coil actin-like protein 6A COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S233} mortality factor 4-like protein 1 COMPARTMENT: cell nucleus MOTIF: MYST1 interaction {26-62} Sufficient for interaction with SIN3A {133-266} MOTIF: nuclear translocation signal {135-146} RB1-1 interaction {164-230} Sufficient for interaction with PHF12 {188-342} RB1-2 interaction {323-344} mortality factor 4-like protein 2 COMPARTMENT: cell nucleus MRG-binding protein COMPARTMENT: cell nucleus MOTIF: glycine-rich region {7-10} YEATS domain-containing protein 4 COMPARTMENT: cell nucleus MOTIF: YEATS {22-126} MLLT10 interaction {163-227} MOTIF: TACC1 interaction {168-227} coiled coil {178-226} YL1 tumor suppressor protein COMPARTMENT: cell nucleus MOTIF: acidic region {22-77} MOTIF: acidic residue (SEVERAL) DNA-binding motif SITE: 156-206 MOTIF: Thr phosphorylation site {T168} proline-rich region SITE: 333-354 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 341-350 MOTIF: proline residue (SEVERAL)

Components

actin actin-like protein 6A; 53 kD BRG1-associated factor A; actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K (ACTL6A, BAF53, BAF53A, INO80K) bromodomain-containing protein 8 (p120, skeletal muscle abundant protein, thyroid hormone receptor coactivating protein 120kD, TrCP120, BRD8, SMAP) DNA methyltransferase 1-associated protein 1; DNMAP1; DNMT1-associated protein 1 (DMAP1, KIAA1425) E1A-binding protein p400; EC=3.6.4.-; CAG repeat protein 32; domino homolog; hdomino; trinucleotide repeat-containing gene 12 protein; p400 kD SWI2/SNF2-related protein (EP400, CAGH32, KIAA1498, KIAA1818, TNRC12) enhancer of polycomb homolog 1 (EPC1) histone acetyltransferase KAT5; 60 kD Tat-interactive protein; Tip60; histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; lysine acetyltransferase 5; cPLA(2)-interacting protein (KAT5, HTATIP, TIP60) inhibitor of growth protein 3 (p47ING3 protein, HSPC301, ING3) mortality factor 4-like protein 1 (MORF-related gene 15 protein, transcription factor-like protein MRG15, MSL3-1 protein, HSPC008/HSPC061, MORF4L1, MRG15, FWP006, PP368) mortality factor 4-like protein 2 (MORF-related gene X protein, transcription factor-like protein MRGX, MSL3-2 protein, MORF4L2, KIAA0026, MRGX) MRG-binding protein (MRGBP, C20orf20) RuvB-like 1 (49-kD TATA box-binding protein-interacting protein, 49 kD TBP-interacting protein, TIP49a, Pontin 52, nuclear matrix protein 238, NMP 238, 54 kD erythrocyte cytosolic protein, ECP-54, TIP60-associated protein 54-alpha, TAP54-alpha, RUVBL1, NMP238, TIP49, TIP49A) RuvB-like 2 (48-kD TATA box-binding protein-interacting protein, 48 kD TBP-interacting protein, TIP49b, repressing pontin 52, Reptin 52, 51 kD erythrocyte cytosolic protein, ECP-51, TIP60-associated protein 54-beta, TAP54-beta, RUVBL2, TIP48, TIP49B, CGI-46) transformation/transcription domain-associated protein; 350/400 kD PCAF-associated factor; PAF350/400; STAF40; tra1 homolog (TRRAP, PAF400) YEATS domain-containing protein 4 (glioma-amplified sequence-41, Gas41, NuMA binding protein 1, NuBI-1, NuBI1, YEATS4, GAS41) YL1 tumor suppressor protein (vacuolar protein sorting protein 72, transcription factor-like 1, protein YL-1, VPS72, TCFL1, YL1)