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NADPH oxidase homolog 1 (NOX-1, NOH-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, mitogenic oxidase 1, MOX1, NOX1, NOH1)
Function:
1) NOH-1S is a voltage-gated proton channel
a) mediates H+ currents of resting phagocytes & other tissues
b) participates in the regulation of cellular pH
c) blocked by Zn+2
2) NOH-1L is a pyridine nucleotide-dependent oxidoreductase
a) generates superoxide
b) conduct H+ ions as part of its electron transport
Cofactor: NADP, FAD
Structure:
- contains 1 ferric oxidoreductase domain, residues 54-545; oxidase activity is potentiated by NOXO1, via residues 397-536
Alternative splicing: named isoforms=3
Expression:
- NOH-1L is expressed in colon, uterus, prostate, colon carcinoma, but NOT in peripheral blood leukocytes
- NOH-1S is detected only in colon & colon carcinoma cells
General
transmembrane 6 protein
Properties
SIZE: MW = 65 kD
entity length = 564 aa
COMPARTMENT: cellular membrane
MOTIF: cytoplasmic domain {1-9}
transmembrane domain {10-30}
exoplasmic loop {31-44}
transmembrane domain {45-72}
cytoplasmic loop {73-102}
MOTIF: Iron [Fe]-binding site
SITE: 101-101
transmembrane domain {103-123}
MOTIF: Iron [Fe]-binding site
SITE: 115-115
exoplasmic loop {124-168}
MOTIF: N-glycosylation site {N162}
transmembrane domain {169-189}
cytoplasmic loop {190-206}
transmembrane domain {207-227}
MOTIF: Iron [Fe]-binding site
SITE: 209-209
Iron [Fe]-binding site
SITE: 221-221
exoplasmic loop {228-396}
MOTIF: N-glycosylation site {N236}
cofactor-binding site [338-344]
COFACTOR-BOUND: flavin adenine dinucleotide
transmembrane domain {397-417}
cytoplasmic domain {418-564}
Database Correlations
OMIM 300225
UniProt Q9Y5S8
PFAM correlations
References
UniProt :accession Q9Y5S8