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nucleolar transcription factor 1; autoantigen NOR-90; upstream-binding factor 1; UBF-1 (UBTF, UBF, UBF1)

Function: - required for transcriptional activator-dependent transcription from TATA-containing promoters [4] - not required for rRNA transcription, but nonetheless DNA transcription is stimulated in the presence of UBF1 [6] - UBF1-stimulated RNA polymerase-1 activity is inhibited by Rb protein [6] - recognizes the ribosomal RNA gene promoter & activates transcription mediated by RNA polymerase 1 through cooperative interactions with the species-specific transcription factor SL1 - binds specifically to the upstream control element - phosphorylated & activated by PIK3CA (putative) - binds to IRS1 & PIK3CA (putative) Structure: - homodimer - contains 6 HMG box DNA-binding domains Compartment: nucleus Alternative splicing: named isoforms=2 Expression: - 50,000 molecules/cell Pathology: - autoantibodies to UBF1 rarely present in patients with scleroderma Laboratory: - upstream-binding factor Ab in serum

Interactions

molecular events

Related

nucleolar organizing region scleroderma (diffuse cutaneous systemic sclerosis)

General

antigen DNA-binding protein phosphoprotein transcription factor (TF)

Properties

SIZE: entity length = 764 aa MW = 89 kD COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl HMG box SITE: 112-180 HMG box SITE: 196-264 MOTIF: Thr phosphorylation site {T201} Ser phosphorylation site {S273} HMG box SITE: 298-362 MOTIF: Ser phosphorylation site {S336} Ser phosphorylation site {S364} Ser phosphorylation site {S389} HMG box SITE: 407-475 MOTIF: Ser phosphorylation site {S412} Ser phosphorylation site {S433} HMG box SITE: 482-549 MOTIF: Ser phosphorylation site {S484} Ser phosphorylation site {S546} HMG box SITE: 568-634 MOTIF: Ser phosphorylation site {S584} Ser phosphorylation site {S638} serine-rich region {675-764} MOTIF: serine residue (SEVERAL) MISC-INFO: COPIES 50000 MOLECULES/CELL

Database Correlations

OMIM 600673 UniProt P17480 Pfam PF00505 Entrez Gene 7343 Kegg hsa:7343

References

  1. Bell et al Science 241:1192 1988
  2. Parisi MA, Clayton DA. Similarity of human mitochondrial transcription factor 1 to high mobility group proteins. Science. 1991 May 17;252(5008):965-9. PMID: 2035027
  3. Weis L, Reinberg D. Transcription by RNA polymerase II: initiator-directed formation of transcription-competent complexes. FASEB J. 1992 Nov;6(14):3300-9. Review. PMID: 1426767
  4. Chiang CM, Roeder RG. Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators. Science. 1995 Jan 27;267(5197):531-6. PMID: 7824954
  5. Dynlacht BD. Retinoblastoma protein. Pol I gets repressed. Nature. 1995 Mar 9;374(6518):114. No abstract available. PMID: 7877677
  6. Cavanaugh AH, Hempel WM, Taylor LJ, Rogalsky V, Todorov G, Rothblum LI. Activity of RNA polymerase I transcription factor UBF blocked by Rb gene product. Nature. 1995 Mar 9;374(6518):177-80. PMID: 7877691
  7. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 1019

Component-of

molecular complex