tRNA (cytosine-5-)-methyltransferase NSUN2 (NOL1/NOP2/Sun domain family member 2, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, substrate of AIM1/Aurora kinase B, NSUN2, SAKI, TRM4)

Function: - RNA methyltransferase - methylates tRNAs, & possibly RNA polymerase 3 transcripts - methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors - not Able to modify tRNAs at positions 48 or 49 - may act downstream of Myc to regulate epidermal cell growth & proliferation - interacts with NPM1 & NCL during interphase interaction is disrupted following phosphorylation at Ser-139 - phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, which inhibits methyltransferase activity & the interaction with NPM1 S-adenosyl-L-methionine + tRNA S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine Structure: - belongs to the methyltransferase superfamily, RsmB/NOP family TRM4 subfamily Compartment: - nucleus, nucleolus, cytoplasm - concentrated in the nucleolus during interphase - distributed in the perichromosome & cytoplasm during mitosis

General

methyltransferase phosphoprotein

Properties

SIZE: MW = 86 kD entity length = 767 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: Ser phosphorylation site {S139} binding site SITE: 184-190 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 215-215 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 242-242 FOR-BINDING-OF: S-adenosylmethionine binding site SITE: 268-268 FOR-BINDING-OF: S-adenosylmethionine cysteine residue {C321} Ser phosphorylation site {S456} Ser phosphorylation site {S724} Ser phosphorylation site {S743} Ser phosphorylation site {S751}

Database Correlations

UniProt Q08J23 Pfam PF01189 ENZYME 2.1.1.29

References

UniProt :accession Q08J23