transcriptional repressor NF-X1; nuclear transcription factor, X box-binding protein 1 (NFX1, NFX2)

Function: - binds to the X-box motif of MHC class II genes & represses their expression - may play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma - isoform 3 binds to the X-box motif of TERT promoter & represses its expression - ogether with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression - mediates E2-dependent ubiquitination - isoform 3 is polyubiquitinated in the presence of HPV16 E6 protein; which leads to proteasomal degradation - isoform 1 is not polyubiquitinated - isoform 1 & isoform 3 interact with human papillomavirus HPV-16 E6 oncoprotein - isoform 1 interacts with PABPC1 & PABPC4 Structure: - the RING-type Zn⁺² finger domain interacts with ubiquitin-conjugating enzyme E2 & facilitates ubiquitination - belongs to the NFX1 family - contains 8 NF-X1-type Zn⁺² fingers - contains 1 R3H domain - contains 1 RING-type Zn⁺² finger Compartment: nucleus Alternative splicing: named isoforms=3 Expression: induced by IFNG/IFN-gamma

General

phosphoprotein ring finger protein transcriptional silencer (transcriptional repressor)

Properties

SIZE: entity length = 1120 aa MW = 124 kD COMPARTMENT: cell nucleus MOTIF: PABPC1 and PABC4 interaction {9-26} Ser phosphorylation site {S49} Ser phosphorylation site {S50} Ser phosphorylation site {S90} RING-finger {358-409} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Zinc finger NAME: Zinc finger SITE: 453-471 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 506-525 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 567-586 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 632-655 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 694-713 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 721-740 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 832-854 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 863-884 EFFECTOR-BOUND: Zn+2 R3H {994-1062} MOTIF: Tyr phosphorylation site {Y1041} proline-rich region SITE: 1084-1089 MOTIF: proline residue (SEVERAL)

Database Correlations

OMIM 603255 UniProt Q12986 PFAM correlations Entrez Gene 4799 Kegg hsa:4799

References

UniProt :accession Q12986