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serine/threonine protein kinase Nek3; HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3 (NEK3)
Function:
- serine/threonine protein kinase
- influences neuronal morphogenesis & polarity through effects on microtubules
- regulates microtubule acetylation in neurons
- contributes to prolactin-mediated phosphorylation of PXN & VAV2
- implicated in prolactin-mediated cytoskeletal reorganization & motility of breast cancer cells through mechanisms involving RAC1 activation & phosphorylation of PXN & VAV2
- prolactin stimulates its activity
- phosphorylation at Thr-479 regulates its catalytic activity
- interacts with PXN, PRLR, VAV1 & VAV2 (prolactin-dependent)
Cofactor: Mg+2
Structure:
- belongs to the protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
- contains 1 protein kinase domain
Compartment:
- cytoplasm (putative)
- cell projection, axon (putative)
Alternative splicing: named isoforms=2
Expression:
- isoform 2 shows a high level of expression in testis, ovary & brain
Pathology:
- up-regulated in breast cancer
General
serine/threonine protein kinase NEK (never in mitosis gene A-related kinase, NimA-related protein kinase, NimA-like protein kinase)
Properties
SIZE: entity length = 506 aa
MW = 58 kD
COMPARTMENT: cytoplasm
STATE: active state
MOTIF: VAV2 interaction {1-285}
MOTIF: kinase domain
SITE: 4-257
MOTIF: ATP-binding site
NAME: ATP-binding site
ATP-binding site
NAME: ATP-binding site
SITE: 10-18
ATP-binding site
NAME: ATP-binding site
SITE: 33-33
aspartate residue {D127}
Thr phosphorylation site {T161}
Ser phosphorylation site {S354}
Ser phosphorylation site {S394}
Thr phosphorylation site {T402}
Thr phosphorylation site {T479}
Database Correlations
OMIM 604044
UniProt P51956
Pfam PF00069
Entrez Gene 4752
Kegg hsa:4752
ENZYME 2.7.11.1
References
UniProt :accession P51956