NEDD4 family-interacting protein 2; NEDD4 WW domain-binding protein 5A; MAPK-activating protein PM04/PM05/PM06/PM07; NF-kappa-B-activating protein 413 (NDFIP2 KIAA1165 N4WBP5A)

Function: - endocytosis - activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 & WWP2, & thus modulates stability of their targets - recruits ITCH, NEDD4 & SMURF2 to endosomal membranes - may modulate EGFR signaling - ubiquitinated by NEDD4 & ITCH - also ubiquitinated by NEDD4L - ubiquitination by NEDD4 or NEDD4L does not affect turnover - undergoes transient Tyr-phosphorylation following EGF stimulation, most probably catalyzed by SRC - phosphorylated by LYN & FYN - forms heterodimers with NDFIP1 - interacts with PTEN - when phosphorylated at Tyr-167, interacts with SRC & LYN SH2 domain - may thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation - interacts with SLC11A2/DMT1 - may interact with phosphorylated EGFR Structure: - the PY (WW-binding) motifs are required for E3 ubiquitin- protein ligase activation & for ubiquitination Compartment: - Golgi membrane, endosome membrane, multivesicular body - multi-pass membrane protein Expression: - expressed in brain, lung, heart, skeletal muscle, kidney, liver, placenta. - induced by T-cell activation

General

phosphoprotein transmembrane 3c protein

Properties

SIZE: entity length = 336 aa MW = 36 kD COMPARTMENT: golgi MOTIF: cytoplasmic domain {1-231} MOTIF: NEDD4 interaction {148-151} PY 1 {149-151} Tyr phosphorylation site {Y151} Tyr phosphorylation site {Y167} Tyr phosphorylation site {Y171} PY 2 {175-177} Tyr phosphorylation site {Y177} PY 3 {184-186} tyrosine residue {186} transmembrane domain {232-252} exoplasmic loop {253-257} transmembrane domain {258-278} cytoplasmic loop {279-287} transmembrane domain {288-308} exoplasmic domain {309-336}

Database Correlations

OMIM 610041 UniProt Q9NV92 Pfam PF10176 Entrez Gene 54602 Kegg hsa:54602

References

UniProt :accession Q9NV92