nuclear distribution protein nudE-like 1 (protein Nudel, mitosin-associated protein 1, NDEL1, EOPA, MITAP,1 NUDEL)

Function: 1) facilitates polymerization of neurofilaments from individual subunits NEFH & NEFL 2) required for organization of the cellular microtubule array & microtubule anchoring at the centrosome 3) regulates microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome (putative) 4) positively regulates activity of minus-end directed microtubule motor protein dynein 5) enhances dynein-mediated microtubule sliding by targeting dynein to microtubule plus ends (putative) 6) required for several dynein- & microtubule-dependent processes, including maintenance of Golgi, centripetal motion of secretory vesicles & coupling of the nucleus & centrosome 7) required during brain development for migration of newly formed neurons from ventricular/subventricular zone toward the cortical plate 8) dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1 9) interacts with YWHAE 10) interacts directly with NEFL & indirectly with NEFH 11) interacts with microtubules 12) self-associate 13) interacts with DISC1, dynein, dynactin, tubulin gamma, KATNA1, KATNB1, PAFAH1B1, PCM1 & PCNT 14) phosphorylated in mitosis 15) can be phosphorylated by CDC2, CDK5 & MAPK1 16) phosphorylation by CDK5 promotes interaction with KATNA1 & YWHAE Structure: - belongs to the nudE family Compartment: - localizes to the cell body of motor neurons - colocalizes with assembled neurofilaments within axonal processes - localizes to microtubules of the manchette in elongated spermatids - cytoplasm - localizes to interphase centrosome & mitotic spindle Alternative splicing: named isoforms=2 Expression: - expressed in brain, heart, kidney, liver, lung, pancreas, placenta & skeletal muscle - expression peaks in mitosis

Properties

SIZE: MW = 38 kD entity length = 345 aa COMPARTMENT: cytoplasm MOTIF: coiled coil {28-190} MOTIF: Self-association {56-166} KATNB1 interaction {64-189} PAFAH1B1 interaction {114-133} interaction with YWHAE {189-256} interaction with NEFL {191-345} MOTIF: KATNB1 interaction {195-256} Ser phosphorylation site {S215} Thr phosphorylation site {T219} DISC1 interaction {241-280} Ser phosphorylation site {S242} FOR-PHOSPHORYLATION-BY: cyclin-dependent kinase-1 Thr phosphorylation site {T245} centrosome & interactions {256-291}

Database Correlations

OMIM 607538 UniProt Q9GZM8 Pfam PF04880

References

UniProt :accession Q9GZM8