homeobox protein NANOG (homeobox transcription factor Nanog, hNanog, NANOG)

Function: 1) transcription regulator involved in inner cell mass & embryonic stem cells proliferation & self-renewal 2) imposes pluripotency on embryonic cells & prevents their differentiation towards extraembryonic endoderm & trophectoderm lineages 3) blocks bone morphogenetic protein-induced mesoderm differentiation of embryonic stem cells by interacting with SMAD1 & interfering with recruitment of coactivators to active SMAD transcriptional complexes 4) transcriptional activator or transcriptional repressor 5) binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' 6) when overexpressed, promotes entry into S phase & cell proliferation 7) interacts with SMAD1 & SALL4 Compartment: nucleus Alternative splicing: named isoforms=2 Expression: - expressed in embryonic stem cell cells - expressed in inner cell mass of the blastocyst & gonocytes between 14-19 weeks of gestation - not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos & early morula - expressed in embryonic stem cells - expression decreases with embryonic stem cell differentiation - expressed in fetal gonads, ovary, testis - not expressed in many somatic organs & oocytes Pathology: 1) expressed in testicular carcinoma & derived germ cell tumors 2) expressed in ovary teratocarcinoma cell line & testicular embryonic carcinoma Gene may be duplicated (10 copies) as pseudogene or retrogene

Properties

SIZE: MW = 35 kD entity length = 305 aa COMPARTMENT: cell nucleus MOTIF: homeodomain SITE: 95-154 consensus repeat {196-200} domain {196-240} MOTIF: transactivation NAME: transactivation SITE: 196-240 consensus repeat {201-205} consensus repeat {206-210} consensus repeat {216-220} consensus repeat {221-225} consensus repeat {226-230} consensus repeat {231-235} consensus repeat {236-240} transactivation NAME: transactivation SITE: 241-305

Database Correlations

OMIM 607937 UniProt Q9H9S0 Pfam PF00046

References

UniProt :accession Q9H9S0