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NADPH oxidase
Function:
- enzyme complex generating the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates
Pathology:
- mutations in the genes for p22phox, p47phox, p67phox & gp91phox are associated with chronic granulomatous disease [3]
Related
chronic granulomatous disease
NADPH oxidase 3; mitogenic oxidase 2; MOX-2; gp91phox homolog 3; GP91-3 (NOX3, MOX2)
General
flavoprotein
molecular complex
oxidoreductase
plasma membrane protein
Properties
COMPARTMENT: plasma membrane
CELL: macrophage
neutrophil
T-cell
B lymphocyte
endosomal membrane
CELL: macrophage
neutrophil
T-cell
B lymphocyte
MOTIF: active site
SUBUNITS: p40phox
MOTIF: PX domain {19-140}
MOTIF: SH3-binding site
NAME: SH3-binding site
Thr phosphorylation site {T154}
src homology 3 [SH3] domain
SITE: 170-229
Ser phosphorylation site {S315}
p47phox
MOTIF: PX domain {4-125}
MOTIF: SH3-binding site
NAME: SH3-binding site
src homology 3 [SH3] domain
SITE: 156-215
acidic region {211-254}
MOTIF: acidic residue (SEVERAL)
src homology 3 [SH3] domain
SITE: 226-285
lysine-rich region {292-390}
MOTIF: lysine residue (SEVERAL)
Ser phosphorylation site {S303}
Ser phosphorylation site {S304}
Ser phosphorylation site {S320}
Ser phosphorylation site {S328}
Ser phosphorylation site {S345}
Ser phosphorylation site {S348}
p67phox
MOTIF: src homology 3 [SH3] domain (2)
rac proto-oncogene protein
MOTIF: active site
P-loop
FOR-BINDING-OF: guanosine triphosphate
guanosine diphosphate
allosteric site
CaaX motif
SITE: C-TERMINUS
QUALITIES: AAX-TRUNCATED
CARBOXYMETHYLATED
EFFECTOR-BOUND: GERANYLGERANYL{C-20}
BOUND-VIA: cysteine residue
cytochrome b558, cytochrome b245, phagocyte/neutrophil B-cytochrome, or flavocytochrome-b
MOTIF: active site
References
- Rotrosen D, Yeung CL, Leto TL, Malech HL, Kwong CH.
Cytochrome b558: the flavin-binding component of the phagocyte
NADPH oxidase.
Science. 1992 Jun 5;256(5062):1459-62.
PMID: 1318579
- Segal AW, Abo A.
The biochemical basis of the NADPH oxidase of phagocytes.
Trends Biochem Sci. 1993 Feb;18(2):43-7. Review.
PMID: 8488557
- Pawson T
Protein modules and signalling networks
Nature 373:573 1995
PMID: 7531822
Components
cytochrome b558
neutrophil cytosol factor 4 (NCF-4, neutrophil NADPH oxidase factor 4, p40-phox, p40phox, NCF4)
p47phox (neutrophil cytosol factor-1, NCF-1, NCF-47K, 47 kD autosomal chronic granulomatous disease protein, Nox-organizing protein 2, SH3 & PX domain-containing protein 1A, NCF1, NOXO2, SH3PXD1A)
p67phox; neutrophil cytosol factor-2; NCF-2; neutrophil NADPH oxidase factor
rac proto-oncogene protein (p21rac)