neural Wiskott-aldrich syndrome protein; N-WASP (WASL)

Function: - regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex - binds to HSF1/HSTF1 & forms a complex on heat shock elements (HSE) that negatively regulates HSP90 expression - binds actin & the Arp2/3 complex - interacts with CDC42 - binds to SH3 domains of GRB2 - interacts with the C-terminal SH3 domain of DNMBP - interacts with the WW domains of PRPF40A/FBP11 (putative) - interacts with NOSTRIN - interacts with Shigella flexneri protein icsA; interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex with maximal activity in actin assembly Structure: - contains 1 CRIB domain - contains 1 WH1 domain - contains 2 WH2 domains Compartment: - cytoplasm, cytoskeleton (putative) - nucleus (putative) - referentially localized incytoplasm when phosphorylated & in nucleus when unphosphorylated (putative)

General

phosphoprotein Wiskott Aldrich syndrome protein family (WASF)

Properties

SIZE: entity length = 505 aa MW = 55 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: WH1 {34-141} CRIB domain {203-216} Tyr phosphorylation site {Y256} proline-rich region SITE: 277-392 MOTIF: proline residue (SEVERAL) WH2 1 {405-422} WH2 2 {433-450} Ser phosphorylation site {S484} Ser phosphorylation site {S485}

Database Correlations

OMIM 605056 UniProt O00401 PFAM correlations Entrez Gene 8976 Kegg hsa:8976

References

UniProt :accession Q9Y6W5